摘要
[ Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. [ Mthod ] The Silurus astus sperms were irradiated by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus. [ Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 rain after fertilization, and cold shock duartion was 40 rain,the survival rate of diploid Silurus astus reached the highest(8.5% ). [ Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files tor further study of gynogenesis development mechanism.
黄河鲇雌鱼较雄鱼生长快、个体大,养殖全雌黄河鲇是提高经济效益的重要途径。该文首次以黄河鲇为材料,用经过紫外线照射处理后的精子激活成熟的卵子,采用冷休克的疗法人工诱导雌核发育二倍体黄河鲇,
基金
the Key Technology Research (0324030026)
Development Program of Henan Province and Key Programs of Zoology of Henan Province~~