摘要
采用水培法,研究Al3+处理下两个油菜品种(耐铝型湘杂油二号和铝敏感型浙双758)叶片的保护酶活性、蛋白质、丙三醛(MDA)和Pro含量。结果表明,Al3+诱导了叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性,在200μmol/L Al3+浓度时活性最大,与处理7 d比较,处理14 d时SOD、CAT活性降低,POD活性增加;此外,叶片膜脂过氧化水平随着Al3+的加重而加剧,在处理14 d时尤其明显,200μmol/L Al3+浓度下,相对耐铝品种湘杂油二号MDA含量比对照增加39%,相对敏感品种浙双758比对照增加52%;同时,叶片脯氨酸含量的提高有助于缓解膜脂过氧化程度,Al3+处理14 d时耐铝品种湘杂油二号的脯氨酸含量明显高于铝敏感品种浙双758。由此说明,铝毒对油菜造成的伤害随着浓度增加和时间延长而加重;浙双758的耐铝性差于湘杂油二号;保护酶活性提高和脯氨酸含量的增加具有缓解铝毒害的作用,但存在一定限度。
A solution culture was conducted to study the effects of aluminium（M） on a tolerant genotype （XZY2） and a sensitive genotype（ZS758） of rape, and the activities of several protective enzymes, the content of protein, MDA or praline in the leaves of rape seedlings were investigated. Results show as follows： The protective enzymes are activated by Al^3＋ treatment and reach the most value under Al^3＋ level of 200 μmol/L; the activities of SOD and CAT in the rape seedlings decrease, but those of POD increase after 14 days comparing to 7 days. Besides, with increase of the Al^3＋ level in the solution culture, significant increase in lipid peroxidation and proline content in the rape seedlings are found especially in the second stage with 14 days. It is indicated that the aluminiurn- stress aggravates the injury for the plant with incearsing the aluminium concentration in solution and the exposure time to aluminium. These protectiase and prohne play an important role in relaxing the toxicity of aluminium, but there is certain limit in the relaxing effect.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2008年第5期107-111,共5页
Journal of the Chinese Cereals and Oils Association
基金
浙江省自然科学基金(307408)
浙江省自然科学基金(306390)项目资助
关键词
铝胁迫
油菜
保护酶
膜脂过氧化
aluminium, rape, protectiase, membrane - lipid peroxidation
