摘要
采用5种蛋白酶对中性蛋白酶A.S1398酶解大豆蛋白生成的相对分子质量高的组分(HMF)进行再次水解制备血管紧张素转化酶(ACE)抑制肽。研究结果表明,Protease N为最适用酶,优化的水解条件为:底物质量分数5%,加酶量2.0%,pH7.0,温度55℃。在此条件下酶解120 min,水解物对ACE抑制作用最强,抑制率达到65.1%(此时水解度为11.1%);水解液经SephadexG-15分离,得到了对ACE具有较好抑制作用的组分,IC50值约为40μg/mL。
The high relative molecular weight fraction （HMF） in soybean protein hydrolysate produced by A. S1398 hydrolysis was further hydrotyzed by 5 kinds of proteases to produce ACE （angioensin Ⅰ - converting enzyme）inhibitory peptides. The results showed that protease N was the most suitable protease, and the hydrolysis conditions were optimized as follows：mass fraction of substrate 5% , enzyme dosage 2.0%, pH 7.0, temperature 55 ℃. Through hydrolyzing for 120 min Under the optimal conditions, the hydrolysates with degree of hydrolysis 11.1% had the most strong ACE inhibitory activity which was up to 65.1%. Fractions with high inhibitory activity were then isolated with gel chromatography. IC50 value of the fractions with the highest inhibitory activity to ACE was about 40 μg/mL.
出处
《中国油脂》
CAS
CSCD
北大核心
2008年第9期6-9,共4页
China Oils and Fats
基金
江苏省农业科技攻关项目"大豆综合深加工关键技术研究及设备开发"(BE2003319)
关键词
大豆蛋白
ACE抑制肽
水解度
凝胶色谱
soybean protein
ACE inhibitory peptides
degree of hydrolysis
gel chromatography
