摘要
采用AFLP标记结合形态学指标对33份萝卜种质的遗传多样性进行评价。筛选出8对能产生稳定可重复片段的引物进行AFLP扩增,每对引物可扩增出35～56条带,平均每对引物扩增的DNA带数为45.2条,其中多态性带占总带数的42.10%,显示出萝卜种质之间存在着较丰富的遗传多样性。基于形态学指标和AFLP标记的聚类分析均可将供试材料分为4大组,其中具有独特紫红色根肉的Rs14独为一组;其余3组的划分,两种方法聚类结果大致相同。从整体来看,萝卜种质分类与根皮色相关,大致可分为绿皮萝卜组、白皮萝卜组、红皮萝卜组。
AFLP and morphological analysis were carried out on 33 radish（Raphanus sativus L. ） germplasms. Eight primer combinations were selected for using in characterizing the radish collection. Differences in number, intensity, and size of amplification products were observed depending on the primer combination, ranging between 35 and 56 bands per pair with an average of 45.2 bands. The polymorphism rate was 42. 10 % , which indicates abundant genetic diversity existing among the cultivated radish germplasms. These germplasms were clustered into four groups by morphological analysis and AFLP, respectively. Rs14 was single in one cluster which suggests it is a unique germplasm for its purple flesh. The clustering patterns of other materials for AFLP and morphological data were similar. And in general, the others were divided into three groups according to their skin colors：green radish, white radish and red radish.
出处
《中国蔬菜》
北大核心
2008年第9期15-18,共4页
China Vegetables
基金
国家科技支撑计划(2008BADA6B05)
山东省良种产业化项目(WKNS200101)
关键词
萝卜
形态学标记
AFLP标记
遗传多样性
Raphanus sativus L. , Morphological markers, AFLP markers, Genetic diversity
