摘要
以龙凤竹〔Pedilanthus tithymaloides(L.)Poit.var.nanus Dressler〕茎段为外植体,研究了龙凤竹愈伤组织诱导、植株再生以及试管苗继代保存培养的培养条件。结果表明,龙凤竹茎段灭菌的最佳方法是用1.0g·L-1HgCl2处理4～10min;愈伤组织诱导与分化的最佳培养基为添加1.5mg·mL-16-BA和0.10～0.15mg·mL-1NAA的MS培养基(含有30g·L-1蔗糖和6g.L-1琼脂粉,pH5.78～pH5.80);试管苗生根的最佳培养基为含有0.2mg·mL-1NAA的生根培养基(1/2MS,含有15g.L-1蔗糖和6g.L-1琼脂粉,pH5.78～pH5.80),试管苗生根率可以达到93.3%;经过炼苗并移栽后,龙凤竹试管苗的成活率可达95.0%以上;龙凤竹试管苗的最佳继代保存培养条件为:在含有0.1mg·mL-1NAA的生根培养基中,于温度15℃、光照强度20μmol·m-2·s-1的条件下继代保存。此外,龙凤竹愈伤组织可以直接分化产生大量丛生芽,达到龙凤竹试管苗增殖的目的。
The culture conditions of callus induction, plantlet regeneration and subculture were studied using stems of Pedilanthus tithymaloides （ L. ） Poit. vat. nanus Dressler as explants. The results show that the optimal sterilization method of stem segments of P. tithymaloides vat. nanus is soaking in 1.0 g ·L^-1 HgCL2 solution for 4 -10 min. The optimal medium of callus induction is MS medium containing 1.5 mg·L^-1 6-BA, 0.10 -0.15 mg·L^-1 NAA, 30 g·L^-1 sucrose and 6 g ·L^-1agar powder （pH 5.78 -pH 5.80）. The optimal rooting medium with rooting rate of 93.3% is the rooting medium （1/2 MS, containing 15 g ·L^-1 sucrose and 6 g ·L^-1 agar powder, pH 5.78 -pH 5.80） containing 0.2 mg ·L^-1 NAA, and the survival rate of P. tithymaloides var. nanus plantlets reach above 95.0% after hardened and transplanted. The optimal subculture medium of P. tithymaloides var. nanus plantlets is the rooting medium containing 0.1 mg ·L^-1 NAA, and the optimal temperature and light intensity in subculture process is 15 ℃ and 20 μmol · m^-2 · s^-1, respectively. Moreover, the callus of P. tithymaloides var. nanus can directly differentiate and form a large amount of rosette buds, so the multiplication goal of P. tithymaloides var. nanus plantlets can be achieved.
出处
《植物资源与环境学报》
CAS
CSCD
2008年第3期73-77,共5页
Journal of Plant Resources and Environment
基金
苏州市农业科技发展计划攻关项目(SNZ-0305)
关键词
龙凤竹
组织培养
愈伤组织
Pedilanthus tithymaloides （L.） Poit. var. nanus Dressier
tissue culture
callus
