摘要
[目的]探讨4℃下标本保存时间对凝血酶原时间、活化部分凝血活酶时间、纤维蛋白原(凝血三项)测定结果的影响。[方法]采血后2h凝血三项测定值分别与采血后4℃下保存8h,12h,24h测定值进行比较,计算两时间段间测定结果的相关系数,作线性回归,求回归方程;把医学决定水平X1,X2,X3分别代入回归方程求Y值,计算各给定的医学决定水平的系统误差,以系统误差小于CLIA 88规定的室间质量评价的总允许误差1/2作为判断标准,以医学决定水平处的系统误差来判断两时间段间测定结果一致性是否为临床可接受。[结果]凝血酶原时间采血后2h与采血后4℃保存8h,12h检测结果具有一致性,为临床可接受,保存24h检测结果为部分接受;活化部分凝血活酶时间采血后4℃保存8h检测结果为临床可接受,保存12h检测结果为部分接受,保存24h检测结果为临床不接受;纤维蛋白原采血后2h与采血后4℃保存8h,12h,24h检测结果具有一致性,为临床可接受。[结论]凝血酶原时间检测,4℃下原始标本可稳定12h;活化部分凝血活酶时间检测,4℃下原始标本可稳定8h;纤维蛋白原检测,4℃下原始标本可稳定24h。
[ Objective] To study the influence of preservation time of samples on measured values of prothrombin time（ PT）activated partial tromboplastin time（APTT）and fibrinogen （FIB） at 4℃. [Methods] The values measured at 2 hours storage time were compared to that stored at 4℃ for 8, 12 and 24 hours respectively. The correlation coefficient was calculated in measured values between two different storage tinie, Then regression equation was made by using linear regression, The medical determination level X1, X2 and X3 were imported to regression equation for calculating Y value. The systemic error（SE） was calculated from given medical determination level, and the clinical acceptability of the consistency was assessed between different measuring time according to the standard of CLIA 88 in which the level of SE was in half of total allowable error in external quality assessment. [Results] The measured values of PT was accepted clinically when samples were stored at 4℃ for 8 and 12 hours because of the consistence of the values measured at different storage time but partly accepted when stored at 4℃ for 24 hours. The value of APTT was accepted clinically when stored at 4℃ for 8 hours but partly accepted when stored at 4℃ for 12 hours and not accepted clinically when stored at 4℃ for 24 hours. The value of FIB was accepted clinically when stored at 4℃ for 8 , 12 and 24 hours. [Conclusions]The measured values of PT was stable for 12 hours,APTT for 8 hours and FIB for 24 hours when .samples were stored at 4℃.
出处
《广西中医学院学报》
2008年第3期8-11,共4页
Journal of Guangxi Traditional Chinese Medical University
基金
2004年广西教育厅科研立项课题(桂教科研NO.200420)
关键词
凝血三项
检测时间
稳定性
临床可接受
Three coagulations
Measure time
Stability
Clinical acceptability
