摘要
目的:研究喉鳞状细胞癌(LSCC)中血管生成素-1,2(Ang-1,2)的表达及与临床病理学特征、CD105标记的微血管密度(MVD)之间的关系,评估Ang-1,2及血管内皮生长因子(VEGF)在LSCC中的联合表达对肿瘤血管生成的作用。方法:用常规免疫组织化学法检测LSCC标本61例、癌旁组织30例及正常喉黏膜组织15例中Ang-1,2及VEGF的表达和MVD,并对临床病理资料进行回顾性分析。结果:Ang-1,2在LSCC细胞、正常喉黏膜上皮细胞及血管内皮细胞均有表达;VEGF只表达于LSCC组织中;LSCC组织与癌旁组织及正常喉黏膜组织相比,Ang-1,2表达显著增加(均P<0.05)。对于临床病理因素的分析表明,Ang-1,2在高、中低分化LSCC中的表达差异无统计学意义(均P<0.05);Ang-2表达与临床分期、MVD有显著的相关性(均P<0.05),而Ang-1的表达与临床分期、MVD无显著的相关性(均P>0.05)。在所有联合表达状态中Ang-2和VEGF同时为阳性的肿瘤组织MVD均值为最高,显著高于其他任何状态(P<0.05)。结论:Ang-1,2在LSCC中的表达可能在肿瘤进展过程中起了重要的作用,与肿瘤血管的形成关系密切。其中Ang-2与VEGF共同作用促进肿瘤血管的形成。
Objective：To investigate the expression of Angiopoietin-l,2 in laryngeal squamous cell carcinoma （LSCC） and the relationship between Angiopoietin-1,2 expression and clinicopathologic parameters and microvessl density （MVD） marked hy CD105, and also evaluate the significance of co-expression of Ang-2 and vascular endo thelial cell growth factor （VEGF） in tumor angiogenesis. Method：The expression of Ang-1,2 and VEGF in samples of tumor, para cancer and normal mucosa were dectected by immunohistochemical method. Result： The expression of Angiopoietin-1,2 were identified in LSCC, para cancer tissue and normal mucosa. The VEGF expres sion was only existed in LSCC. The expression of Ang 1,2 were significantly higher in LSCC than in para cancer tissue （P〈0. 05） and normal mucosa （P〈0. 01 ）. In the clinicopathologic parameters, only the expression of Ang-2 was closely correlated with clinical stages and MVD （all P〈0.05）. There was no significant correlation between the expression of Ang 1,2 and tumor differentiation degree （all P〉0.05）. When the expression of Ang-2 and VEGF were both positive, the mean value of MVD was higher than others（P〈0.05）. Conclusion： These results suggest that overexpression of Ang-1,2 may play a very important role in the development of LSCC and are closely correlated with angiogenesis. Ang 2 promote angiogenesis when interacting with VEGF.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第17期772-776,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
血管生成素-1
血管生成素-2
血管内皮生长因子
喉肿瘤
癌
鳞状细胞
血管生成
Angiopoietin 1
Angiopoietin-2
Vascular endothelial growth factor
Laryngeal neoplasms
Larynx squamous cell carcinoma
Angiogenesis
