摘要
应用S—P免疫组织化学方法研究62例(男性51例,女性11例,年龄34~68岁,平均年龄53.7岁)肺癌组织中P16蛋白和P53蛋白的表达情况,并进行增殖细胞核抗原(PCNA)检测,计算细胞增殖指数(Proliferationindex,PI)。62例肺癌组织中P16蛋白和P53蛋白阳性率分别为58.1%和59.7%。腺癌P16蛋白的阳性率明显高于小细胞癌(P<0.05);淋巴结转移阳性组P16蛋白的表达显著低于阴性组(P<0.05);PI分级为Ⅱ级的P16蛋白表达显著高于Ⅳ级(P<0.05)。不同组织类型肺癌中P53蛋白的表达未见明显差异,淋巴结转移阳性组P53蛋白的表达高于阴性组(P〈0.01);不同PI分级中P53蛋白的表达,Ⅳ级明显高于Ⅰ级(P〈0.05)和Ⅱ级(P〈0.05),Ⅲ级明显高于Ⅰ级(P〈0.05)和Ⅱ级(P〈0.05)。P16蛋白低表达和P53蛋白过表达之间未见明显相关。揭示P16蛋白低表达和P53蛋白过表达均有促进肺癌细胞增殖的作用,P16蛋白的表达与肺癌的细胞分化有关,P53蛋白过表达对肺癌细胞的转移起重要作用。抑癌基因P53对MTS1/P16基因无明显调控作用,检测P53蛋白表达?
By using S-P immunohistochemical methods,the expression of p16 and p53 proteins in 62 cases of human lung carcinoma were studied.The positive expression for p16 and p53 protein were 58.1%and 59.7% respectively.A significant correlation was found between p16 protein lowexpression,p53 protein overexpression and lymph node metastasis(P<0.05).Meanwhile,a significant correlation was found between p16 protein lowexpression、p53 protein overexpression and cell proliferation index(P<0.05).No significant correlation between the expression of p16 and p53 protein was found.The results suggested that p16 protein lowexpression and p53 protein overexpression played an important role in cell proliferation of lung carcinoma.
基金
江西省人民医院科研基金