P^(53)突变产物在睑板腺癌中的增强表达及其意义

Over-expression of P^(53) mutant protein in meibomian gland carcinoma

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摘要我们采用免疫组化S－P法，对17例石蜡包埋睑板腺癌及癌旁组织标本的P53突变蛋白表达进行了检测。结果：17例癌组织有12例表达阳性，阳性率71％。其中分化型7例，6例呈阳性（86％）表达。在一组原发灶及淋巴结转移癌配对标本中，同一病人之两个部位P(53)突变蛋白表达差异无明显意义。癌旁（睑缘）组织17例中5例上皮有异常增生，且同时伴有P(53)突变蛋白呈强阳性表达。以上结果提示：抑癌基因P53的突变在睑板腺癌的发生中是一个比较常见的基因改变，且在分化型癌的发生中表现明显；P(53)基因异常发生在肿瘤转移之前且在淋巴道转移中可能起重要作用；P(53)突变蛋白在癌旁上皮异型增生组织中的过度表达具有十分重要的意义。 By using SP immunohistochemical method, we studied the expression of P(53) protein in paraffin blocks of 17 cases of meibomian gland carcinoma and their adjacent margo palpebrae tissues. The results of immunostaining showed that 12 cases (71%) of meibomian gland carcinoma were positive. In 7 cases of differentiated type, 6 cases were positive. No significant difference was found on the expression of P(53) protein between primary and metastatic sites in 3 paired samples of meibomian gland carcinoma. The expression of P53 protein were strong positive in 5 cases adjacent margo palpebrae in which there were dysplastic squamous epithelium in the base. The results suggest that anti-oncogene P53 may be one of genetic events which occur often in the development of meibomian gland carcinoma and it was presented apparently in carcinoma of differentiated type. The abnormal of P53 gene had occurred before the lymph node metastasis of tumor. Over-expression of P53 proteinhas deep significance in abnormal prolifertion of adjacent epithelium tissues.