摘要
目的：建立止喘口服液中盐酸麻黄碱的含量测定法。方法：采用高效液相色谱法，选用Kromasil C18（250mm×4．6mm，5μm）色谱柱；流动相为乙腈-0．1％磷酸溶液（10：90，V／V）；检测波长：207nm；流速0．7mL／min；进样量20tzL；柱温：室温。结果：盐酸麻黄碱在4．5—27．0mg／L范围内线性关系良好（r=0．9998，n=6），平均回收率为98．99％，RSD为0．96％。结论：本法简便快捷、灵敏，结果准确，可用于止喘口服液中盐酸麻黄碱的质量控制。
Objective：To develop a method for the determination of ephedrine hydrochloride in Zhichuan oral solution. Methods： HPLC method was used. The column was Kromasil C18（250 mm ×4.6 mm, 5 μm）. The mobile phase was acetonitrile-0.1% phosphoric acid solution （10： 90, V/V ）. The detection wavelength was 207 nm. The flow rate was 0.7 mL/min and the injection volume was 20 p.L. Results： The liner range of ephedrine hydrochloride was 4.5 - 27.0 mg/L （ r = 0. 9998, n = 6 ）. This average recovery was 98.99% and RSD was 0.96%. Conclusions： This method is simple, rapid, sensitive and accurate, and can be used as a method of quality control for ephedrine hydrochloride in Zhiehuan oral solution.
出处
《儿科药学杂志》
CAS
2008年第5期40-42,共3页
Journal of Pediatric Pharmacy
关键词
高效液相色谱法
盐酸麻黄碱
止喘口服液
High performance liquid chromatography
Ephedrine hydrochloride
Zhichuan oral solution
