摘要
目的建立HPLC同时测定双青咽喉片中枸橼酸、没食子酸和绿原酸含量的方法。方法色谱柱:Dikma Kromasil C18柱(4.6mm×250mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;分段变波长测定;柱温:30℃;流速:0.8mL.min-1。结果枸橼酸、没食子酸和绿原酸的线性范围分别为:0.6024～6.024μg(r=0.9999),0.0312～0.312μg(r=0.9999),0.2016～2.016μg(r=0.9999),平均回收率分别为98.56%,99.13%,98.26%,RSD为0.95%,0.78%,1.37%。结论该方法简便、快速、灵敏、准确、具有良好的重复性和回收率,可作为该制剂的质量控制标准。
OBJECTIVE To establish a HPLC method for the simultaneous determination of citric acid, gallic acid and chlorogenic acid in Shuangqing Yanhou tablets. METHODS The separation was carried out on a Dikma Kromasil C18 column（4. 6 mm ×250 mm, 5um） with gradient elution. The mobile phase was acetonitrile-0. 1% phosphoric acid solution. The samples were measured at multiple wavelengths. The column temperature was 30 ℃. The flow rate was 0. 8 mL·min^-1. RESULTS The calibration curves were linear in the ranges of 0. 602 4 -6. 024ug（r =0. 999 9） for citric acid,0. 031 2 -0. 312 ug（r =0. 9999） for gallic acid,0. 201 6 -2. 016 ug （r =0. 999 9）for chlorogenic acid. The average recoveries were 98.56% with RSD 0. 95% for citric acid,99. 13% with RSD 0. 78% for gallic acid,98.26% with RSD 1.37% for chlorogenic acid. CONCLUSION This method is simple,rapid,sensitive,accurate and with good repeatability and recovery, it can be used as a quantitative analysis for this preparation.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2008年第19期1507-1510,共4页
Chinese Pharmaceutical Journal
基金
泸州市重点科技项目(泸市科〔2004〕25号)
关键词
枸橼酸
没食子酸
绿原酸
含量测定
citric acid
gallic acid
chlorogenic acid
content determination
