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HPLC法测定喜树幼枝中喜树碱含量 被引量:6

HPLC determination of camptothecin in young shoots of Camptotheca acuminata Decne
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摘要 目的:建立 HPLC 法测定喜树幼枝中喜树碱的含量,并用该方法测定14个种源地喜树幼枝中喜树碱的含量。方法:采用 VP-ODS 色谱柱(150 mm×4.6 mm,5μm)为色谱柱,甲醇-水(62:38)为流动相,流速为1.0 mL·min^(-1),检测波长为254nm,柱温为25℃。结果:喜树碱进样量在0.10~1.00μg范围内线性关系良好(r=0.9999)。结论:本法测定喜树幼枝中喜树碱含量操作简便,结果准确可靠。 Objective:To establish an HPLC method for determining of camptothecin in young shoots of Camptotheca acuminata Decne. Method:VP- ODS column(150 mm ×4. 6 mm,5 μm) was used and the mobile phase consisted of methanol -water(62: 38) at the flow rate of 1.0 mL · min^- 1;The determination wavelength was 254 nm and 25 ℃ for column. Results : The linear range was 0. 10 - 1.00 μg ( r = 0. 9999 ). Conclusion : This method is simple and the result is accurate and reliable for determining of camptothecin.
出处 《药物分析杂志》 CAS CSCD 北大核心 2008年第9期1535-1537,共3页 Chinese Journal of Pharmaceutical Analysis
基金 山西省运城学院院级项目(20060233)
关键词 高效液相色谱法 喜树 喜树碱 幼枝 含量 HPLC Camptotheca acuminata Decne. camptothecin young shoot contents
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