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Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a‘Wangshuibai’derived population 被引量：1

Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a 'Wangshuibai' derived population
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摘要 A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we used a ＇Wangshuibai＇ （resistant）/＇Seri82＇ （susceptible） derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ＇Wangshuibai＇ at 96 h after inoculation was 2.6 times higher than ＇Seri82＇. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat. A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we used a ＇Wangshuibai＇ （resistant）/＇Seri82＇ （susceptible） derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ＇Wangshuibai＇ at 96 h after inoculation was 2.6 times higher than ＇Seri82＇. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
作者 Amir Mohammad Naji Mohammad Moghaddam Mohammad Reza Ghaffari Hashem Pour Irandoost Laleh Karimi Farsad Seyed Mostafa Pirseyedi Seyed Abolghasem Mohammadi Behzad Ghareyazie Mohsen Mardi
机构地区 Department of Agronomy and Crop Breeding Department of Agronomy and Plant Breeding Department of Genomics
出处 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第10期625-629,共5页 遗传学报（英文版）
基金 Agricultural Biotechnology Research Institute of Iran (Iranian Ministry of Ji-had-e-Agriculture)
关键词 EST-derived STS markers Qfhs.ndsu-3BS Fusarium head blight WHEAT EST-derived STS markers Qfhs.ndsu-3BS Fusarium head blight wheat
分类号 S512.1 [农业科学—作物学]
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  • 1Aarts, M.G., Lintel Hekkert, B., Holub, E.B., Beynon, J.L., Stiekema W.J., and Pereira, A. (1998). Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana. Mol. Plant Microb. Interact. 11: 251-258.
  • 2Anderson, J.A., Stack, R.W., Liu S., Waldron, B.L., Fjeld A.D., Coyne, C., Moreno-Sevilla, B., Mitchell Fetch, J., Song, Q.J., Cregan, P.B., and Frohberg, R.C. (2001). DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor. Appl. Genet. 102: 1164-1168.
  • 3Bai, G.H., Kolb, F.L., Shaner, G.E., and Domier, L.L. (1999). Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89: 343-348.
  • 4Bai, G.H., and Shaner, G.E. (2004). Management and resistance in wheat and barley to Fusarium head blight. Annu. Rev. Phytopathol. 42: 135-161.
  • 5Bassam, B.J., and Caetano-Anollés, G. (1993). Silver staining of DNA in polyacrylamide gels. Appl. Biochem. Biotechnol. 42: 181-188.
  • 6Buerstmayr, H., Lemmens, M., Hartl, L., Doldi, L., Steiner, B., Stierschneider, M., and Ruckenbauer, P. (2002). Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. Ⅰ. Resistance to fungal spread (type Ⅱ resistance). Theor. Appl. Genet. 104: 84-91.
  • 7Buerstmayr, H., Steiner, B., Hartl, L., Griesser, M., Angerer, N., Lengauer, D., Miedaner, T., Schneider, B., and Lemmens, M. (2003). Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. Ⅱ. Resistance to fungal penetration and spread. Theor. Appl. Genet. 107: 503-508.
  • 8Chee, P.W., Rong, J.K. Williams-Coplin, D., Schulze, S.R., and Paterson, A.H. (2004). EST derived PCR-based markers for functional gene homologues in cotton. Genome 47: 449-462.
  • 9Chen, R.H., Sarnecki, C., and Blenis, J. (1992). Nuclear localization and regulation of the erk- and rsk-encoded protein kinases. Mol. Cell. Biol. 12: 915-927.
  • 10Collins, N.C., Webb, C.A., Seah, S., Ellis, J.G., Hulbert, S.H., and Pryor, A. (1998). The isolation and mapping of disease resistance gene analogs in maize. Mol. Plant Microb. Interact. 11: 968-978.

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Journal of Genetics and Genomics
2008年 第10期

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