摘要
目的建立一种血清尿素测定的新方法和临床应用。方法应用脲酶、丙酮酸氧化酶、丙酮酸激酶与色原系统2,4-二氯苯酚、4-氨基安替比林,进行血清尿素浓度的酶法测定。结果磷酸盐缓冲液最适浓度为50mmol、pH7.2,最大吸收峰为510nm,线性范围为0～42mmol/L,显色稳定。精密度:批内(n=20)CV为1.89%～3.21%,批间(n=20)CV为1.96%～3.45%,回收率为96.2%～104.2%,平均回收率为99.5%。与酶偶联速率法比较,相关系数r=0.995,回归方程^Y=1.023x+0.38,两法高度相关,正常参考值范围为儿童组2.20～8.50mmol/L,少年组2.60～8.60mmol/L,成人组2.85%～8.95mmol/L。结论本法具有高度的特异性和准确性,其操作简便、灵敏快速、标本用量少、结果稳定,适用于全自动、半自动生化分析仪,更适用于中小医院,有较大的推广使用价值。
Objective To establish a new assay method for serum urea determination, and explore its clinical application. Methods Urease, pyruvate oxidase, pyruvate kinase and aniline pigment system 2,4-diehlorophenol, and 4-amino-antipyrine were applying in enzymic method assay for serum urea. Results The optimal concentration of phosphate buffer was 50 mmol, pH value was 7.2, maximum absorption peak was at 510 nm, and the linearity range was 0-42 mmol/L; precisicn： within-run （n=20） CV=1. 89%-3.21% and between-run （n=20） CV= 1.96%-3.45%; the recovery rate was 96.2%-104.2%, with an average of 99.5%. As compared with enzyme coupled rate method, the coefficient＇correlation was 0. 995, regression equation was Y= 1. 023x＋ 0.38, and the two methods were highly correlated. Normal reference value range was 2. 2-8. 5 mmol/L in children group, 2. 6-8. 6 mmol/L in juvenile group, and 2.85-8.95 mmol/L in adult group. Conclusion The new assay method is simple, sensitive, rapid and result stable with high specificity and accuracy, which is applicable in complete automatic, semi-automatic biochemical analysis instrument. It is suitable for spread and use in mediumor small-sized hospital.
出处
《国际检验医学杂志》
CAS
2008年第9期792-793,795,共3页
International Journal of Laboratory Medicine
