摘要
目的探讨眼发育调控基因Pax6在人视网膜母细胞瘤中的表达情况。设计实验性研究。研究对象新鲜视网膜母细胞瘤肿瘤组织6例,正常视网膜组织6例,视网膜母细胞瘤细胞株2例。方法采用逆转录酶链反应(RT-PCR)、Western蛋白印迹方法检测视网膜母细胞瘤肿瘤组织中Pax6 mRNA和Pax6蛋白的表达,以正常视网膜组织作为对照。同时检测SO-Rb50、Y79视网膜母细胞瘤细胞株中Pax6 mRNA和Pax6蛋白的表达情况。主要指标视网膜母细胞瘤新鲜肿瘤组织及细胞株中Pax6基因的表达。结果RT-PCR和Western蛋白印迹检测显示,在6例视网膜母细胞瘤肿瘤组织及两种视网膜母细胞瘤细胞株中均可见Pax6 mRNA及Pax6蛋白表达。与正常视网膜组织相比较,视网膜母细胞瘤肿瘤组织中Pax6 mRNA及Pax6蛋白表达水平增高(P均<0.01)。结论Pax6基因的异常表达可能参与并导致人眼视网膜母细胞瘤的发生和发展。
Objective To detect the gene expression of Pax6 in retinoblastoma (Rb). Design Experimental study. Participants Six cases of fresh Rb tumors and two established Rb cell lines. Six cases of retina tissues as normal control group. Methods Pax6 gene expression was assessed in two established Rb cell lines, six primary tumors and six cases of retina tissues using the reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot. Main Outcome Measures Expression of Pax6 gene in fresh retinoblastoma tissue and cell lines. Results The mRNA of Pax6 gene was positive in all two established Rb cell lines and the six primary Rb tumors using RT-PCR. The protein of Pax6 gene was also positive in Western blot. Pax6 mRNA and protein expression in the Rb tumors were increased compared with normal control group (P〈0.01). Conclusion These date support a role for the PAX family in oncogenesis, by identifying another human neoplasm in which they are inappropriately expressed. Pax6 expression in retinoblastoma may contribute to pathogenesis by supporting cellular proliferation in the de-differentiated state.
出处
《眼科》
CAS
2008年第5期352-356,共5页
Ophthalmology in China
基金
国家自然科学基金项目(30772377)