摘要
探讨红车轴草提取物(RCE)在体外对小鼠T淋巴细胞和巨噬细胞的影响。MTT法检测RCE对细胞的毒性作用。荧光抗体染色结合流式细胞术检测RCE对T淋巴细胞在Con A的刺激下表达活化抗原CD69、CD25、CD71的影响。CFDA-SE标记技术结合流式细胞术分析RCE对T淋巴细胞在Con A诱导下增殖情况的影响。Griess法检测RCE对小鼠巨噬细胞在LPS刺激24 h后分泌NO的影响。RCE对小鼠有潜在的抗炎作用。RCE对小鼠淋巴细胞和巨噬细胞的细胞毒作用很小。不同质量浓度的RCE能够很好的抑制过量的炎症相关信号分子表达,如NO,CD69,CD25,CD71,且呈剂量依赖性。RCE能够抑制T淋巴细胞的增殖。数据显示RCE可能通过对小鼠淋巴细胞活化与增殖及巨噬细胞NO分泌的抑制展示其抗炎效应。
The study investigated the effects of red clover extract （RCE） on mouse T macrophages and lymphocytes in vitro. The cell toxic effect of RCE was estimated by MTT assay. Multiple-fluorescence staining plus flow cytometry were used to detect the effect of RCE on CD69/CD25/CDT1 expression of mouse T lymphocytes stimulated by Con A; CFDA-SE staining plus flow cytometry were used to analyze the effect of RCE on proliferation of T lymphocytes activated by Con A; The effect of RCE on nitric oxide （NO） secretion of mouse macrophages stimulated by lipopolysaccharide （LPS） for 24 h was assayed by Griess reagent system. We found that RCE had potent anti-inflammatory effects on mice. RCE had little cell toxic effect on mouse lymphocytes and macrophages. RCE strongly inhibited the excessive production of inflammatory mediators （NO, CD69, CD25, CD71 ）, in a dose-dependent manner, like cyclosporine A injection. RCE could inhibit proliferation of CD3^＋ T lymphocytes. These data suggested that RCE might exhibit anti-inflammatory effect by inhibiting the activation and proliferation of mouse lymphocytes and the NO secretion of mouse macrophages.
出处
《药学学报》
CAS
CSCD
北大核心
2008年第10期1019-1024,共6页
Acta Pharmaceutica Sinica
基金
"973"National Basic Research Program of China(2006CB504201,2004CB720100)
the Key Sci-tech Development Project of Guangzhou (2006Z-E0091)
Natural Foundation of Guangdong Province(2006B36030016).
