摘要
采用毒性实验方法,用不同浓度的汞离子(Hg2+)、铬离子(Cr6+)分别处理黄鳝(Monopterus albus),经1、2、4、8d后光镜观察黄鳝头肾组织结构及免疫细胞数量的变化。结果表明,对照组黄鳝头肾实质内未见肾单位结构,主要由淋巴组织、造血组织和血窦构成。淋巴组织主要由淋巴细胞密集排列成淋巴索或聚集成团状;造血组织主要由红血细胞密集排列成细胞索或聚集成团状。淋巴组织和造血组织在头肾实质中各占据一定的区域或相间分布,且分别被血窦所分隔。黑色素巨噬细胞分散于淋巴细胞之间,数量较少,排列较松散,尚未集结成明显的球状体。经两种重金属离子分别染毒后的黄鳝头肾与对照组相比,组织结构表现出相似的变化,即随着重金属离子浓度的增加和染毒时间的延长,头肾组织中的黑色素巨噬细胞聚集形成黑色素巨噬细胞中心,并逐渐增大、增多,最后减少;淋巴组织逐渐松散,排列稀疏混乱,淋巴细胞界限逐渐不清晰,呈退化趋势,数量减少。红血细胞大量破坏,血窦扩张。与Cr6+相比,Hg2+对头肾组织结构的毒性影响更大,病变现象更为明显。
Based on the toxicological experimental method, the changes of microscopic structures and the number of immune cells of head-kidney of the Ricefield Eel （ Monopterus albus ） were investigated after the fish was exposed separately under different concentrations of mercury ions （ Hg^2＋ ） and the chromium ions （ Cr6^2＋） on day 1,2,4,8. The results showed that： in control, head-kidney parenchyma consisted of the lymphatic tissue, hematopoietic tissue and sinusoid, nephrons were absent. The lymphatic tissue was primary composed of lymphocytes which crowdedly arranged to the lymphocyte rope or gathered the group. The hematopoietic tissue mainly consisted of the red blood cells which crowdedly arranged to the cell rope or gathered the group. Lymphatic tissue and hematopoietic tissue respectively occupied the certain region or interval distributed, and separated by sinusoids. The less melano-macrophage that scattered between the lymphocytes loose arranged, not formed obvious spherical body. Comparing with the control, histological structure of fishes exposed to two kinds of heavy metal ions displayed similar changes. Along with increasing of the heavy metal ion concentration and lengthening of the treatment time, melano-macrophages gathered melano-macrophage centre, and gradually increased in the number and distribution, then reduced. Lymphatic tissue gradually loosed,the arrangement was sparse and chaotic. The lymphocytes border was unclear, the number gradually reduced, and showed retrogressive tendency. The red blood cells were massively damaged, sinusoid was expanded. The influence of Hg^2＋ on histological structures of head-kidney of the Ricefield Eel was greater than Cr^2＋ , and the pathological phenomenon was more obvious too.
出处
《动物学杂志》
CAS
CSCD
北大核心
2008年第5期120-127,共8页
Chinese Journal of Zoology
基金
福建省泉州市科技计划项目(No.2004N24)
福建省教育厅科技计划项目(No.JA06015)
关键词
黄鳝
铬离子
汞离子
头肾
组织结构
Monopterus albus
Cr^2＋
Hg^2＋
Head-kidney
Histological structure
