摘要
为了提高兰花炭疽病拮抗菌巨大芽孢杆菌(Bacillus megaterium)1-12菌株的芽孢形成率及芽孢数量,在摇瓶发酵基础上对芽孢形成的主要影响因素进行了考察.通过单因素实验和正交实验对1-12菌株产芽孢条件进行了分析,确定了摇瓶发酵最佳条件:培养基组成(质量分数)为玉米粉1%,大豆蛋白胨1%,CaCl2.2H2O 0.1%,MnSO4.H2O 0.05%;培养液初始pH 6.0;培养条件为种龄20 h,装瓶量30 mL/250 mL,接种量8%,温度30℃,摇瓶转速200 r/min.在此条件下,B.megaterium1-12菌株发酵液中的芽孢数量为1.92×109个mL-1,芽孢形成率达到了95%.
In order to improve the spore formation rate and spore quantity of antagonistic strain 1-12 of Bacillus megaterium against Colletoichum gloeosporioides, the main factors influencing spore formation were investigated using shake-flask fermentation method. Through single factor experiment and orthogonal experi- ment, the optimal shaking flask fermentation conditions were determined as follow： medium composed （mass fraction） of 1% corn flour, 1% soybean peptone, 0.1% CaCl2· 2H2O, 0.05 % MnSO4·H2O. Initial pH 6.0, incubation time 20 h and flask coutent 30 mL / 250 mL, 8% inoculum size, fermentation temperature 30℃, rotating speed 200 r/min. Under this optimum conditions, 1.92 × 10^9 mL 1 were obtained, and the spore formation rate achieved 95 %.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2008年第5期522-528,共7页
Journal of Hebei University(Natural Science Edition)
基金
引进国际先进林业科学技术创新项目“花卉新品种创制技术引进与创新”(2006-4-C08)
关键词
兰花炭疽病
胶胞炭疽菌
拮抗细菌
巨大芽孢杆菌
芽孢
发酵
Orchid anthracnose
Colletoichum gloeosporioides
antagonistic bacteria
Bacillus megaterium
spore
fermentation
