摘要
目的研究脑源性神经营养因子（BDNF）及酪氨酸激酶B（TrkB）受体在大鼠海马结构CA1区时空分布变化。方法采用免疫组织化学实验，观察BDNF及TrkB在正常大鼠发育期、成年期和老龄期海马结构CA1区的定位分布及时间变化。结果BDNF，TrkB在海马结构的时空表达基本一致。生后0d，CA1颗粒层上片染色较深，细胞轮廓隐约可见。成年期，细胞染色增强，轮廓清晰，核大而圆无着色；老年期，阳性细胞数量开始下降，染色变淡，CA1锥体阳性细胞层变薄，染色淡。细胞轮廓不清，阳性细胞发生了明显退行性变。结论海马结构内BDNF，TrkB主要表达于CA1区的锥体细胞层，表达高峰均为生后15～20d。老年期呈明显的增龄性下降，阳性细胞发生退行性变化。
Objective To study systematically the distribute of BDNF and TrkB in the normal rat hippocampal formation CAt. Methods Observes systematically the expression changes of brain-derived neurotrophic factor （BDNF） and tyrosine kinase receptor （TrkB） in the normal rat brains during development and adulthood and aged phase. Results BDNF and TrkB immunoreactive neurons appeared in cells of the pyramidal cell layer on postnatal day （PND） 0. The immnuostaining intensity reached a plateau-like pattern during adulthood. The number of immnuoreaacting cells decreased during aged phase. Pyramidal cell layer of CA1 became thinner. BDNF aad TrkB were expressed significantly in immnuoreaacting cells during adulthood. During aged phase,immnuoreaacting cells slightly degenerated. Conclosion The expression of BDNF and TrkB reached a peak in hippocampal formation CA1 of rats on PND 20. The expression of BDNF and TrkB decreased gradually after adulthood. During aged phase,the expression of BDNF and TrkB increased significantly, and the form of the cell occurred degeneration.
出处
《现代检验医学杂志》
CAS
2008年第5期70-71,共2页
Journal of Modern Laboratory Medicine
基金
陕西省卫生厅科研基金项目(No06D-12)
