摘要
目的建立田基黄中槲皮素-7-O-α-L-鼠李糖苷的含量测定方法，为田基黄药材的质量控制和综合开发利用提供一定的科学依据。方法采用HPLC法。色谱柱为Hypersil C18柱（250mm×4．6mm，5μm），流动相为体积分数0．04％的乙腈-磷酸水（体积比77：23），流速为1．0mL·min^-1，检测波长为371nm，柱温为35℃。结果槲皮素-7-O-α-L-鼠李糖苷质量浓度在3．12～99．84mg·L^-1（r=0．9998，n=6）内与峰面积呈良好的线性关系；平均回收率为99．1％（RSD=1．5％，n=9）。结论该方法简便、快速、精密度好，可用于田基黄的质量控制。
Objective To establish an HPLC method for the determination of quercetin-7-O-α-L-rhamnoside in Hypericum japonicum. Methods The separation was performed on an Hypersil C18 column （250 mm×4.6 mm, 5μm） with the mobile phase consisting of 0.04 % acetonitrile-aqueous phosphoric acid （ V ： V = 77：23）at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 371 nm and the column temperature was set at 35 ℃. Results The calibration curve was linear over the range of 3.12 - 99.84 mg·L^-1 for linarin （ r = 0. 999 8）. The average recovey was 99.1% （RSD = 1.5 %, n = 9）. Conclusions The method is simple, accurate and reproducible, which is suitable for the quality control of Hypericum japonicum.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2008年第10期806-809,共4页
Journal of Shenyang Pharmaceutical University
