摘要
目的:采用病、证模型复合的方法建立大鼠肾亏型颈椎病动物模型。方法:选择3月龄雌性SPF级SD大鼠30只,随机分为正常组、颈椎病模型组和肾虚型颈椎病模型组,每组10只。采用动静力失衡性大鼠颈椎间盘退变模型复合去卵巢肾虚亏模型的方法建立肾虚型颈椎病模型。通过检测大鼠子宫及附件形态和质量、血清雌二醇(estradiol,E2)含量验证肾虚证;通过颈椎间盘组织病理学、Ⅱ型和Ⅹ型胶原的免疫组化检测及聚集蛋白聚糖(aggrecan-1,Agc1)、Ⅱ型前胶原基因(typeⅡprocolla-gen gene,Col2a1)、基质金属蛋白酶-13(matrix metalloproteinase-13,MMP-13)和基质金属蛋白酶抑制剂-1(tissue inhibitor of metalloproteinases-1,TI MP-1)的基因表达情况评判椎间盘退变。结果:与正常组和颈椎病模型组相比,肾虚型颈椎病模型组动物子宫及附件形态萎缩、质量减轻,血清雌二醇含量降低,颈椎间盘组织病理学退变更加明显,Ⅱ型胶原蛋白表达减少,Ⅹ型胶原表达增高,Agc1和Col2a1基因表达降低,MMP-13基因表达增高。结论:通过病、证模型复合的方式建立了肾虚型颈椎病模型,肾虚可以加重颈椎间盘的退变。
Objective：To establish a rat model of cervical syndrome（CS） with kidney deficiency.Methods：A group of30three-month-old female Sprague-Dawley rats were randomly divided into normal control group,CS group and CS with kidney deficiency group（combined group）,with10rats in each group.Rats in the normal control group received no treatment,rats in the CS group underwent only resection of cervical muscles and ligaments as unbalanced dynamic and static animal model,and rats in combined group underwent resection of both cervical muscles and ovaries as kidney deficiency model.Serum and cervical intervertebral discs were collected.Kidney deficiency was determined by observing the morphologic changes of uterus and appendages,detecting the weight of uterus and appendages and the content of serum estradiol（E2）.The degeneration of intervertebral discs was determined by detecting the histopathology,the expressions of typeⅡcollagen and typeⅩcollagen proteins,and the expressions of aggrecan-1（Agc1）,typeⅡprocollagen gene（Col2a1）,matrix metalloproteinase-13（MMP-13） and tissue inhibitor of metalloproteinases-1（TIMP-1） mRNAs.Results：Compared with those in the normal control group and CS group,the rats in the combined group were noted with the uterus atrophied,the caliber of oviduct thinned,the weight of uterus and appendages diminished obviously,the content of serum E2decreased,cervical intervertebral disc degenerated more seriously,typeⅡcollagen protein expression decreased,typeⅩcollagen protein expression increased,Agc1and Col2a1mRNA expressions in intervertebral disc decreased,and the MMP1-3mRNA expression increased.Conclusion：The rat model of CS with kidney deficiency is established.Kidney deficiency can aggravate cervical intervertebral disc degeneration.
出处
《中西医结合学报》
CAS
2008年第10期1034-1039,共6页
Journal of Chinese Integrative Medicine
基金
国家杰出青年科学基金(No.30625043)
国家自然科学基金重大国际合作项目(No.30710103904)
国家自然科学基金(No.30572398
30600829&30701118)
上海市中药现代化重点项目(No.06DZ19718)
上海市教育委员会创新基金(No.08YZ56)
关键词
肾虚
颈椎病
病证结合
动物疾病模型
大鼠
kidney deficiency, cervical syndrome, combining disease and syndrome, disease model,animal, rats
