摘要
为了探索硒对妊娠早期子宫局部细胞因子IL-2、IL-4、TGF-β1及TGF-β2的调节作用，将妊娠山羊分为试验Ⅰ、Ⅱ、Ⅲ组和对照组。Ⅰ、Ⅱ、Ⅲ组山羊分别以0．3、0．5和0．7mg／kg剂量的硒灌服亚硒酸钠溶液，对照组灌服1mL／kg的蒸馏水，每周1次，连续3周。用ELISA试剂盒检测子宫局部细胞因子IL-2、IL-4、TGF-β1及TGF-β2的动态变化以观察硒对其分泌的调节作用。结果表明：（1）妊娠早期（15～19d）山羊两侧子宫角中IL-2、IL-4、TGF-β1及TGF-β2水平无显著差异。妊娠早期山羊两侧子宫角的子宫液IL-2水平在15～18d呈上升趋势，19d时趋于下降；IL-4水平一直呈上升趋势；TGF-β1水平较高，呈不规律变化；TGF-β2呈现先升高、后趋于平稳的趋势。（2）灌服亚硒酸钠溶液，可以显著降低妊娠16～18d子宫液IL-2的水平（P〈0．05或P〈0．01）；同时可显著升高IL-4的水平（P〈0．05或P〈0．01），且呈一定的剂量依赖关系。（3）0．5和0．7mg／kg剂量组显著升高妊娠16～18d子宫液TGF-β1水平（P〈0．05或P〈0．01）；同时对妊娠16～17d子宫液TGF-β2水平也有显著的升高作用（P〈0．05或P〈0．01），但对18d时的TGF-β2影响不显著。试验表明，硒对妊娠早期山羊子宫局部IL-2、IL-4、TGF-β1及TGF-β2的分泌具有一定的调节作用。
In order to explore the regulation of the local-uterine cytokine IL-2, IL-4, TGF-β1 and TGF-β2 by selenium（Se） during early gestation of goats, the pregnant goats were grouped into experimental groups Ⅰ、Ⅱ、Ⅲ and control group. Group Ⅰ、Ⅱ and Ⅲ were treated with sodium selenite respectively at doses of 0.3, 0.5 and 0.7 mg（Se）/kg by oral administration weekly for three times and control group was similarly treated with distilled water. Uterine samples were collected to determine the content of IL-2, IL-4, TGF-β1 and TGF-β2 by ELISA. The results showed that （1）There were no differences at the levels of all the tested cytokine between two uterine horns of each goat during early gestation. The IL-2 levels in fluid of two uterine horns increased during 15-18 d after pregnancy and began to decrease on day 19 after pregnancy, and IL- 4 remained in a continuously high level during 15-19 d after pregnancy. The TGF-β1 had irregular high level in the same period and TGF-βz increased primely and then remained at a high level. （2） Administration of 0.3, 0. 5 and 0.7 mg/kg selenium could significantly decrease the content of IL-2 in uterine fluid during 16-18 d after pregnancy and significantly increase the level of IL-4 in uterine fluid in a dose-dependent manner. （3）Goats of 0.5 and 0.7 mg/kg groups showed significantly high level of TGF-β1 in uterine fluid during 16-18 d after pregnancy, and presented high level of TGF-β2 in uterine fluid during 16 17 d after pregnancy but no significant effect on TGF-β2 secretion on day 18 after pregnancy. These results indicated that selenium has a regulatory function on secretion of the local-uterine IL-2, IL-4, TGF-β1 and TGF -β2 during early pregnancy in goats.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第10期1432-1437,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(39970544)
关键词
硒
山羊
妊娠
细胞因子
selenium
goats
pregnancy
cytokine
