摘要
以新型含有磷酸胆碱基的仿细胞膜两亲聚合物——胆固醇封端的聚(2-甲基丙烯酰氧基乙基磷酸胆碱)(CPMPC)为表面稳定剂实现碳纳米管的表面改性,利用两亲聚合物中的胆固醇疏水段与碳纳米管表面进行非共价键的稳定结合,通过两亲聚合物中聚(2-甲基丙烯酰氧基乙基磷酰胆碱)(PMPC)亲水段实现其水溶性和生物相容性.并以商业可获得的典型两亲分子,末端为胆固醇的聚氧乙烯(CPEG)和卵磷脂,为对照进行研究.研究表明CPMPC和CPEG均具有比卵磷脂更高的对碳纳米管进行分散的能力.而CPMPC改性的碳纳米管比CPEG改性的碳纳米管具有更优的稳定性和生物相容性,通过新型仿细胞膜聚合物改性的碳纳米管在生物医用领域有潜在应用.
A novel biomimetic amphiphilic polymer, cholesterol-end-capped poly（2-methacryloyloxyethyl phosphorylcholine） （CPMPC）,was used as surfactant to achieve water soluble and biocompatible carbon nanotubes （CNTs）. Crude CNTs were facilely dispersed in aqueous media by uhrasonication with the help of CPMPC, with cholesterol-end-capped PEG （CPEG） and lecithin as controls. The ability of the novel biomimetic amphiphile to solubilize and stabilize the CNTs was investigated by UV spectra. The solubility of CNTs in 1 mg/mL CPMPC and CPEG aqueous solutions was found to be 1. 705 mg/mL and 1.468 mg/mL, respectively. The solubility of CNTs, in 0.8 mg/mL lecithin was, however, only 0. 326 mg/mL. Furthermore, CPMPC coated CNTs precipitated quite slowly compared with CPEG coated CNTs, which indicated better stabilizing ability of CPMPC. Cell culture was used to evaluate the biocompatibility of the amphiphiles stabilized CNTs using MTF assay and cell morphology observation. While CPEG coated CNTs showed obviously detrimental effect on the growth of HUVEC304 cells, CPMPC coated CNTs did not exhibit obvious toxicity. The biocompatible CPMPC coated CNTs represent an excellent nano-object for potential biomedical applications.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2008年第10期1006-1009,共4页
Acta Polymerica Sinica
基金
国家自然科学基金(基金号20774082
50703036)
国家高技术研究发展计划(863计划
项目号2006AA03Z329
2006AA03Z444)
浙江省科技计划项目(项目号2007C24008)
教育部"新世纪优秀人才计划"(项目号NCET-05-0527)资助
关键词
碳纳米管
磷酸胆碱
细胞膜仿生
生物相容性
Carbon nanotubes, Phosphorylcholine, Biomimetic, Biocompatibility
