摘要
[目的]探索黄瑞香组织培养和快速繁殖的培养条件。[方法]以黄瑞香幼嫩茎尖为外植体,采用植物组织培养和生物统计的方法研究黄瑞香的组织培养和快速繁殖技术。[结果]确定黄瑞香茎尖诱导愈伤组织的最佳培养培养基为WPM+IBA 0.1 mg/L+KT 0.1mg/L,茎尖诱导芽的适宜培养基为WPM+IBA 0.2 mg/L+6-BA 3 mg/L,幼芽形成根的适宜培养基为1/2 MS+IBA 3.0 mg/L。[结论]该研究结果为黄瑞香的快速繁殖及工厂化生产奠定了基础,该研究为黄瑞香组织培养和快速繁殖提供依据。
[ Objective ] The study aimed to explore the condition for tissue culture and rapid propagation of Daphne giraldii Nitsch. [ Method] Taking young stem tip of Daphne giraldii Nitsch. as explants, plant tissue culture and biostatistics methods were used to study the tissue culture and rapid propagation technique of Daphne giraldii Nitsch. [ Result] The optimum medium for callus induction was WPM ＋ IBA 0.1 mg/L ＋ KT 0.1 mg/L. The appropriate medium for the shoot induction and the rooting were WPM ＋ IBA 0.2 mg/L ＋ 6-BA 3 mg/L and 1/2 MS ＋ IBA 3.0 rag/L, respectively. [ Conclusion] The research results laid a foundation for the quick propagation and factory production of Daphne giraldii Nitsch, and offered references for the tissue culture and rapid propagation of Daphne giraldii Nitseh.
出处
《安徽农业科学》
CAS
北大核心
2008年第26期11226-11227,11229,共3页
Journal of Anhui Agricultural Sciences
基金
河南大学自然科学基金(06YBZR079)资助
关键词
黄瑞香
组织培养
培养基
Daphne giraldii Nitsch.
Tissue culture
Medium
