期刊文献+

丹参提取物764—3对新生大鼠高氧所致急性肺损伤的保护作用 被引量:2

Protective effect of Radix Salviae Milliorrhizae Extract (764-3) on hyperoxia-induced acute lung injnry of neonatal rats
原文传递
导出
摘要 目的探讨丹参提取物764.3对高氧暴露所致新生大鼠急性肺损伤的保护作用,并将其作用与超氧化物歧化酶(SOD)进行比较。方法采用新生spragueDawley大鼠置于≥90% O2中7d以建立高氧急性肺损伤模型,分别用SOD和764—3进行干预,对比肺系数、丙二醛(MDA)含量、SOD活性、辐射状肺泡计数(RAC)和病理形态学变化,并采用脱氧核糖核酸转移酶介导的细胞凋亡标记技术法(TUNEL)检测肺细胞凋亡情况。结果SOD干预组和764.3干预组在高氧暴露7d时的MDA含量和凋亡细胞数均低于高氧对照组(P〈0.05或P〈0.01),病理形态学改变要轻于高氧对照组,RAC及SOD活性则要高于高氧对照组(P〈0.05或P〈0.01),且两种抗氧化剂作用近似(P〉0.05)。但764—3干预组的肺水肿与高氧对照组差异无显著性(P〉0.05)。结论764—3对新生大鼠高氧所致的急性肺损伤有一定的保护作用。 Objective To study the protective effect of Radix Salviae Milliorrhizae Extract (764-3) on hyperoxia-induced acute lung injury of neonatal rats and to compare the effect of it with that of superoxide dismutase (SOD). Methods Hyperoxic lung injury model was established by putting Sprague Dawley neonatal rats into self-made oxygen box within high-concentration oxygen(≥90 % O2)for 7 days. Interfered with SOD and 764-3 respectively, the lung index, contents of malondialdehyde (MDA), activity of SOD, radical alveolar counts (RAC) and pathological changes have been compared. Meanwhile, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was utilized to detect the number of apoptosis of lung ceils. Results On the seventh day, the contents of MDA and the number of apoptosis of lung cells of SOD prevention group and 764-3 prevention group were lower than those of the hyperoxia-exposed group ( P 〈 0.05 or P 〈 0.01 ), while RAC and activity of SOD were higher(P 〈 0.05 or P 〈 0.01 ) ; pathological changes of apoptosis of lung cells of SOD prevention group and 764-3 prevention group were leas than those of the hyperoxia-exposed group, The protective effect of SOD and 764-3 were similar(P 〉 0.05 ). However, compared with those of hyperoxia-exposed group, the lung index of 764-3 prevention group showed insignificant difference(P 〉 0.05). Conclusion Radix Salviae Milliorrhizae Extract (764-3) may have certain protective effect on hyperoxia-indueed acute lung injury.
出处 《中国小儿急救医学》 CAS 2008年第5期458-461,共4页 Chinese Pediatric Emergency Medicine
关键词 高浓度氧 急性肺损伤 丹参提取物 大鼠 Hyperoxia Acute lung injury Radix Salviae Milliorrhizae Extract Rat
  • 相关文献

参考文献8

  • 1郭子健,朱元珏.中药764—3对内毒素所致离体大鼠灌流肺损伤的保护作用探讨[J].中国应用生理学杂志,1995,11(3):225-228. 被引量:10
  • 2Kinsella JP, Greenough A, Abman SH. Bronchopulmonary dysplasia. Lancet, 2006, 367 (9520) : 1421-1431.
  • 3Geiser T, Jarreau PH, Atabai K, et al. Interleukin-1 augments in vitro alveolar epithelial repair. Am J Physiol Lung Cell Mol Physiol, 2000, 279(6) : L1184-1190
  • 4Gerik SM, Keeney SE, Dallas DV, et al. Neutrophil adhesion molecule expression in the developing neonatal rat exposed to hyperoxia. Am J Respir Cell Mol Biol, 2003, 29 (4) : 506.
  • 5周卫辉,陈祥银,余丽君,赵青,严仪昭.尼古丁对中性粒细胞活化及细胞间粘附分子基因表达的影响[J].中国病理生理杂志,2000,16(9):779-782. 被引量:10
  • 6Buckley S, Barsky L, Weinberg K, et al. In vivo inosine protects alveolar epithelial type 2 cells against hyperoxia-induced DNA damage through MAP kinase signaling, Am J Physiol Lung Cell Mol Physiol, 2005, 288(3) : L569-L575.
  • 7De Paepe ME, Mao QF, Chao Y, et al. Hyperoxia-induced apoptosis and Fas/FasL expression in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol, 2005, 289 (4) :L647-L659.
  • 8Oh SH, Nan JX, Sohn DW, et al. Salvia miltiorrhiza inhibits biliary obstruction-induced epatocyte apoptosis by cytoplasmic sequestration of p53. Toxicol Appl Pharmacol, 2002, 182 ( 1 ) : 27- 33.

二级参考文献2

共引文献18

同被引文献23

引证文献2

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部