摘要
运用人类高分辨染色体显微切割、ＰＣＲ和微克隆技术构建的８ｑ２４．１带特异性ｐＵＣ１９文库，筛选出一个含ＣＡ重复的新的多态微卫星ＤＮＡ［（ＣＡ）ｎ］。经ＰＣＲ检测人鼠杂种细胞板，证实其来源于人８号染色体；在正常人群中检出１１个等位片段，杂合度为０．８４；在１１个家系中进行连锁分析。结果显示，此多态标记与三个已知的位于８ｑ２３－２４．１区域的微卫星ＤＮＡ（Ｄ８Ｓ８５，Ｄ８Ｓ１９９，Ｄ８Ｓ２８１）紧密连锁，其在８号染色体遗传图谱上的相对位置为：着丝粒－Ｄ８Ｓ８５－新（ＣＡ）ｎ－Ｄ８Ｓ１９９－长臂末端。
A clone, containing a 24dinucleotide(CA)repeat sequence, was isolated from chromosome 8q24.1 bandspecific pUC19 library. It was localized to target chromosome using humanrodent hybrid cell panel. Eleven alleles were estimated from 56 unrelated individuals and the heterozygosity reaches 0.835. Linkage analysis of 11 Chinese pedigrees showed significant evidence for linkage of this new marker to three dinucleotide repeat markers at 8q2324.1 region, with the most likely position of the marker between D8S85 and D8S199.
出处
《湖南医科大学学报》
CSCD
1997年第6期471-474,共4页
Bulletin of Hunan Medical University
关键词
微卫星DNA
遗传学
医学
染色体
microsatellite repeats
genetics,medical
linkage
chromosomes,human
