摘要
应用ＥＬＩＢ技术观察旋毛虫肌蚴抗原（ＴｓＭＬＳＡ）与６种寄生虫病人血清的交叉反应。结果表明：ＴｓＭＬＳＡ中３１～１００ＫＤａ内的蛋白大部分可被急性血吸虫病人血清所识别，而慢性血吸虫病人血清仅识别其中的４４／４５，５１／５３，６２／６４及１００ＫＤａ蛋白；小于６０ＫＤａ者可与丝虫病、钩虫病、肺吸虫、蛔虫病和肝吸虫病人血清呈不同程度的交叉反应，以前三者为最明显；４５ＫＤａ蛋白可被部分正常人血清识别。提示，旋毛虫肌蚴抗原与多种寄生虫有共享成分，但与血吸虫的交叉可依据４组区带用以鉴别诊断此二种寄生虫病。
ross reactions of T. spiralis muscular larva antigens (TsMLA) against several sera from patients with other parasitic diseases by ELIB (enzymelinked immunoblotting technique) were studied. The proteins of 31100KDa were recognized by sera of two cases of acute schistosomiasis, while proteins of 44/45, 51/53, 62/64 and 100KDa were recongnized by sera of 11 cases of chronic schistosomiasis. The proteins of 60KDa showed varied cross reactions against sera of five kinds of patients with other parasitic diseases (filariasis, hookworm diseases, paragonimiasis, ascariasis and clonorchiasis). Only the proteins of 45KDa were recognized by six of fifteen cases of normal human sera. All of >29KDa proteins were recognized by sera of rabbit infected by T. spiralis. It is suggested that there were many antigens in Trichinellen muscular larva which shared in common with several other parasites. The cross reactions of TsMLA against Schistosoma japonicum may be differentiated on the positive reaction bands of 44/45, 51/53, 62/64 and 100KDa proteins which was specific to the chronic schistosomiasis using the TsMLA by means of ELIB.
出处
《湖南医科大学学报》
CSCD
1997年第6期475-477,共3页
Bulletin of Hunan Medical University
基金
世界银行贷款资助
关键词
日本血吸虫
血吸虫病
旋毛虫病
抗原
交叉反应
Schistosoma japonicum
schistosomiasis
Trichinella spiralis
antigen
helminth
cross reaction
rats
