摘要
目的探讨镍污染地区大气细颗粒物(PM2.5)对大鼠肺泡巨噬细胞吞噬功能的影响。方法选择某镍污染地区及非污染地区,监测环境空气质量、采集颗粒物并进行镍浓度分析。将42只成年雄性 SPF 级 Wistar 大鼠随机分为生理盐水对照组、镍污染区组和非污染区组,镍污染区组和非污染区组又分为颗粒物低(1.6 mg/kg)、中(8.0 mg/kg)、高(40.0 mg/kg)剂量组,每个剂量组6只。一次性气管滴注染毒颗粒物,24 h 后处死大鼠,观察大鼠肺泡灌洗液中肺泡巨噬细胞吞噬率、吞噬指数的变化。结果镍污染地区与非污染地区环境空气质量水平相似,镍污染地区大气颗粒物中镍浓度是非污染地区的48.75倍。毒理实验结果显示,与生理盐水对照组比较,镍污染区组巨噬细胞吞噬率在低剂量即显著降低(P<0.05),而非污染区组则在中剂量显著性降低(P<0.05);相同剂量下镍污染区组吞噬率均比非污染区组低,在高剂量(40.0mg/kg)时差异有统计学意义(P<0.05)。吞噬指数变化趋势相似。结论镍污染地区大气 PM2.5对大鼠肺泡巨噬细胞的毒性效应大于非污染区,其中镍可能起到了关键性作用。
Objective Through the comparative study of the acute toxic effects of PM2.5 in nickel-contaminated area in rats to explore the possible mechanism of acute toxicity of PM2.5. Methods A nickel-contaminated area and a control area were selected, the air quality of the two areas was monitored and the concentration of nickel in the collected PM2.5 was determined. Forty two male Wistar rats （SPF Grade） were randomly divided into three groups, including one saline control group and two experimental groups, the nickel-polluted group and the non-polluted group, in each. There were three subgroups. PM2.5 were administered to rats by intratracheal instillation at doses of 1.6 mg/kg, 8.0 mg/kg and 40.0 mg/kg respectively. The rats were sacrificed 24 hours after treatment and the brochoalveolar lavage fluid （BALF） were collected, the phagocytic function of alveola macrophages of rats were tested. Results There was no significant difference between the level of environmental quality in the two areas, the concentration of Ni in PM2.5 in the nickel-polluted area was 48.75 times of that in the non-polluted control area. The phagocytic function of alveola macrophages of rats in the experimental groups decreased significantly （P〈0.05） compared with the saline control group. The phagocytic function of alveola maerophages of rats in the nickel polluted area decreased significantly compared with the non-polluted control area （P〈0.05）. Conclusion Nickel may play a key role in the acute toxicity of PM2.5 in the nickel-polluted area.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第10期864-866,共3页
Journal of Environment and Health
关键词
空气污染
巨噬细胞
颗粒物
Air pollution
Maerophages
Particulate matter
