摘要
目的测定大卫颗粒中黄芩苷的含量。方法采用高效液相色谱法,色谱柱为C18柱（4.6mm×250 mm,5μm）,流动相为甲醇-0.2%磷酸（50∶50）检测波长280 nm。结果黄芩苷在1.504-15.04μg范围内呈线性,平均回收率为99.04%,RSD=0.87%（n=6）。结论本方法简便、灵敏,准确,可用于大卫颗粒的质量控制。
Objective To establish the determination method of baicalin in Dawei Keli. Methods The HPLC method for baicalin was used. Column：ODS-C18 （4. 6 mm ×250 mm ,5μm） , mobile phase：methanol ：0. 2% H3 PO4 （50：50）, UV wave length ：280 nmo Result There was a good linear relationship in the range of 1. 504 - 15. 04μg, The average recovery was 99.04% , RSD was 0. 87% （n = 6 ）. Conclusion This method is simple, accurate and reliable. It can be used for the quality control of Dawei Keli .
出处
《中国实用医药》
2008年第27期49-50,共2页
China Practical Medicine
