摘要
目的:建立一种常用的气相色谱法分离检测ICH中规定的34种残留溶剂。方法:以DB-624弹性石英毛细管柱为色谱柱(75 m×0.53 mm×3.0μm),载气为氮气,采用氢火焰离子化检测器(FID),进样口温度为220℃,检测器温度为300℃,柱温采用程序升温(35℃保持18 min;以5℃·min^(-1)升至45℃,保持8 min;以5℃·min^(-1)升至80℃,保持3 min;以5℃·min^(-1)升至150℃,保持2 min;再以30℃·min^(-1)升至250℃,并保持5 min),流速3 mL·min^(-1)。以N-甲基吡咯烷酮为溶剂,进样体积为1μL。结果:34种有机溶剂均能得到很好的分离,分离度绝大多数均大于1.5。峰面积与浓度呈良好的线性关系,精密度良好。结论:本文气相色谱条件可用于常用的34种溶剂的分离与测定,方法简单准确。
Objective:To establish a CGC method for determination of 34 residual solvents in ICH ,like methanol, ethanol, ether and ect.. Method: Using a DB - 624 column (75 m× 0.53 mm × 3.0 μm) ; Flame ionization detector (FID) ;The injector temperature was 220 ℃ ;The detector temperature was 300 ℃ ;The column temperature was programmed,the initial temperature was 35 ℃ ,maintain for 18 min,raise to 45 ℃ with a rate of 5 ℃ ·min^-1, maintain for 8min, raise to 80 ℃ with a rate of 5 ℃ · min^- 1, maintain 3 min, raise to 150 ℃ with a rate of 5 ℃ . min^ - 1, maintain for 2 min, then raise to 250 ℃ with a rate of 30 ℃ · min^ -1, maintain for 5 min ; Nitrogen was used as carrier gas, the flow rate was 3 mL · min^ - 1. NMP as the dissolution solvent. Injection : 1 μL. Results: The calibration curves of each solvent had good linear relationship, and those solvents could be completely separated. Conclusion:The established mathod is accuracy,sensitive,simple and can be used in detection of residual solvents.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第10期1767-1770,共4页
Chinese Journal of Pharmaceutical Analysis