摘要
为了明确缺氮条件下甜菜NR的活性,用活体测定法检测了经缺氮胁迫不同时间后甜菜叶中NR活性.结果表明,随着缺氮处理时间的延长,甜菜叶中的NR活性逐渐降低,在缺氮处理1 h后,其活性下降较快.用50 mmol/L KNO3溶液处理的甜菜幼苗总RNA,通过RT-PCR分离得到了硝酸还原酶基因片段,长度为471 bp,Blast分析表明,其与Genbank中硝酸还原酶基因部分序列具有高度同源性.
The experiments are conducted to investigate the activity of nitrate reductase in sugar beet （Beta vulgaris L. ） under different time of N - absent treatment. The results show that the activity of NR rapidly decrease in the first 1 h and gradually decrease when the time of N - absent treatment is prolonged. The homolog cDNA with 471 base pairs of nitrate reduetase gene is obtained by RT - PCR from total RNA using Beta vulgaris treated by 50 mmoL/L KNO3 as material. Blast analysis showed high identity to part sequence of Beta vulgaris nitrate reductase gene.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2008年第5期610-613,共4页
Journal of Harbin University of Commerce：Natural Sciences Edition
基金
国家自然科学基金资助项目(30571096)
东北林业大学青年基金资助项目(07038)
