摘要
应用反相高效液相色谱法测定了4，4’-二羧酸-2，2’-联喹啉（又名2，2’-联喹啉酸，简写作BCA），作为BCA生产过程的质量控制方法。方法中采用Phenomenex-C18柱（4．6mm×200mm，5μm）作为固定相，采用甲醇与0．05mol·L^-1乙酸钠溶液，以3比7的体积比混合配成的溶液作为流动相，其流速为0．5mL·min^-1，柱温为28℃，在330nm波长处作UV-检测，所测得的峰面积值与BCA质量浓度在0．05-10．0mg·L^-1之间呈线性关系。从同一批产品中取7份试样，按所提出的方法测定其BCA的含量，测得BCA量的平均值为98．62％，7次测定的相对标准偏差为1．65%。
Reverse-phase HPLC was applied to the determination of 4, 4＇-dicarboxy-2, 2＇-hiquinoline （or named 2,2＇-bicinchoninic acid, abbreviate as BCA） as a method of quality control in course of its production. The phenomenex-C18 column （4.6 mm × 200 mm, 5μm） was used as the stationary phase and a mixture of methanol and 0. 05 mol · L^-1 NaOAc solution, mixed in the ratio of 3 to 7 by volume, was used as the mobile phase at a flow-rate of 0. 5 mL · min^-1 , and with column temperature of 28 ℃. UV-detection was made at the wavelength of 330 nm. Linear relationship between values of peak area and concentration of BCA was obtained in the range of 0. 05 - 10. 00 mg · L^-1. Seven sample of BCA taken from product of same batch, and contents of BCA were determined by the proposed method. The average content of BCA found was 98. 62%, with value of RSD （n=7） to be 1.65%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2008年第10期952-953,共2页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
湖南省教育厅资助课题(04C346)
