摘要
本实验采用雄性大鼠胸主动脉环,研究灯盏花素舒张血管的机制。结果表明,灯盏花素(1×10-6~1×10-3mol/L)对去甲肾上腺素(1×10-6mol/L)的收缩反应呈剂量依赖性的松驰作用,与内皮无关;也不受普萘洛尔(1×10-5mol/L)所影响。灯盏花素能拮抗去甲肾上腺素诱发的依外钙与依内钙的双相反应,而且对后者的抑制作用较强。结果提示,灯盏花素诱发血管舒张可能与受体操纵性钙通道和细胞内Ca2+释放的抑制有关。在高钾所致的主动脉环收缩反应中,灯盏花素在有Ca2+的Krebs液中表现出兴奋作用,不受酚拉明(1×10-5mol/L)影响;而在无Ca2+的Krebs液中灯盏花素能使CaCl2的剂量反应曲线压低,其机制仍有待于研究。
The relaxation effects of Breviscapine on isolated thoracic aorta rings of rats were observed. The results indicated that Breviscapine ( 1×10 -6  ̄1×10 -3 mol/L) could make the NE-induced vasoconstriction relaxed in a concentration-dependent manner. That was independent to endothelium and couldn't be influenced by 1×10 -5 mol/L of propranolol. Breviscapine could antagonize the NE induced biphase contractive responses which were dependent to extracellular and intracelluar calcium, but the antagonization effect for the latter was stronger than that for the former. The results suggested that the vasodilation effect of Breviscapine might be related to the inhibition of the receptor operated calcium channel and intracellular Ca 2+ release. Breviscapine had an exciting action for the high K +evoked contraction of the aorta rings in the normal Kreb′s solution,that can′t be influenced by phentolamine,but lowered the dose response curve of CaCl 2 in the calcium free pre incubation Kreb′s solution.The mechansim for this phenomenen remains being studid.
出处
《广东药学院学报》
CAS
1997年第4期215-218,共4页
Academic Journal of Guangdong College of Pharmacy
关键词
灯盏花素
三羟基黄酮
葡萄糖酸甙
胸主动脉环
Breviscapine
noradrenaline
verapamil
phentolamine
isoprenaline
propranolol
KCl
CaCl 2
thoracic aorta rings