摘要
目的筛选对于大麻多态性好的AFLP引物标记来区分大麻品种。方法利用AFLP技术用55对引物组合对12个大麻地域品种进行初筛。结果选出5对多态性好的引物组合进行了遗传多样性研究。每对AFLP引物组合扩增出47～76条带,共获得285条带,其中多态性条带为99条以及10条品种特异带。结论AFLP对于大麻具有很高的分辨率,为今后深入地研究大麻植物遗传多样性奠定了很好的基础。
Objective To screen the AFLP primers with good diversity to distinguish various species of Cannabis. Methods The AFLP was used to analyze the genetic diversity of 12 species of Cannabis using 55 primer combinations. Results A total of 285 AFLP bands were obtained using five primer combinations with better diversity, among which 99 bands were polymorphie and 10 bands were special, with 47-76 bands amplified in each pair of primers. Conclusion AFLP may has good resolution in the diversity study of Cannabis. It may provide an essential basis for further study of the genetic diversity of Cannabis.
出处
《法医学杂志》
CAS
CSCD
2008年第5期330-332,共3页
Journal of Forensic Medicine
基金
国家十一五支撑计划项目基金资助(2006BAK09B02)
