摘要
研究了共振散射技术测定甲拌磷残留的新方法。实验结果表明，酸性溶液中，甲拌磷和钨酸钠相互作用能够形成杂多酸，该杂多酸与碱性三苯甲烷染料罗丹明B（RhB）结合，形成了缔合物粒子，导致体系在λex/λem=606nm共振散射（RLS）强度急剧增强，并产生新的RLS光谱。在选定的实验条件下，甲拌磷浓度在0．43-200nmol／L范围内，与体系的RLS强度有良好的线性关系（r=0．9977），据此建立了共振散射法快速测定甲拌磷的新方法，该法检出限为0．057nmol／L，回收率为82％～99％，用于蔬菜样品中甲拌磷残留量的测定，与气相色谱法对照，结果满意。
A novel method for determination of phorate residue was studied by resonance light scattering（RLS） technique, based on the formation of the association particles between rhodamine B and heteropoly acides produced by the reaction of phorate with sodium tungstate in acid medium. The experimenal results indicated that the great increase of RLS at λex/λem=606 nm was observed. A good linear relationship （ r = 0. 997 7） was exhibited for phorate concentration in the range of 0. 43 - 200 nmol/L with the detection limit of 0. 057 nmol/L. The recoveries were range of 82% - 99% . The method was applied to determine the concentration of phorate residue in vegetable. The obtained results using RLS method were well in agreement with those obtained by gas chromatography method.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第10期1062-1066,共5页
Journal of Instrumental Analysis
关键词
缔合物粒子
共振散射
甲拌磷残留
钨酸钠
罗丹明B
association particle
resonance light scattering
phorate residue
sodium tungstate
rhodamine B
