摘要
AIM: To explore the antitumor bioactivity of adenovirus- mediated mutant type p27kip1 gene in a colorectal cancer cell line SW480. METHODS: We constructed recombinant adenovirus vector expressing a mutant type p27kip1 gene (ad- p27mt), with mutation of Thr-187/Pro-188 (ACGCCC) to Met-187/Ile-188 (ATGATC), and transduced into SW480 cells. Then we detected expression of p27, Bcl-2 and Bax protein in the transductants by Western blotting, cell cycle of transductants by a digital flow cytometric system, migrating potential with Boyden Chamber and SW480 tumor cell growth inhibition in vitro and in vivo. RESULTS: We found that a recombinant adenovirus vector of expressing ad-p27mt, with mutation of Thr-187/Pro-188 (ACGCCC) to Met-187/Ile-188 (ATGATC) has potent inhibition of SW480 tumor cell growth in vitro and in vivo. Furthermore, ad-p27mt induced cell apoptosis via regulating bax and bcl-2 expressions, and G1/S arrest in SW480 cells and inhibited cell migration. CONCLUSION: ad-p27mt has a strong anti-tumor bioactivity and has the potential to develop into new therapeutic agents for colorectal cancer.
AIM： To explore the antitumor bioactivity of adenovirusmediated mutant type p27^kip1 gene in a colorectal cancer cell line SW480. METHODS： We constructed recombinant adenovirus vector expressing a mutant type p27^kip1 gene （ad- p27mt）, with mutation of Thr-187/Pro-188 （ACGCCC） to Met-187/Ile-188 （ATGATC）, and transduced into SW480 cells. Then we detected expression of p27, Bcl-2 and Bax protein in the transductants by Western blotting, cell cycle of transductants by a digital flow cytometric system, migrating potential with Boyden Chamber and SW480 tumor cell growth inhibition in vitro and in vivo. RESULTS： We found that a recombinant adenovirus vector of expressing ad-p27mt, with mutation of Thr-187/Pro-188 （ACGCCC） to Met-187/Ile-188 （ATGATC） has potent inhibition of SW480 tumor cell growth in vitro and in vivo. Furthermore, ad-p27mt induced cell apoptosis via regulating bax and bcl-2 expressions, and G1/S arrest in SW480 cells and inhibited cell migration. CONCLUSION： ad-p27mt has a strong anti-tumor bioactivity and has the potential to develop into new therapeutic agents for colorectal cancer.
基金
Grant from the Natural Science Foundation of Hubei Province, NO.2003ABA193
