摘要
从珊瑚中来源的各种红色荧光蛋白(red fluorescent protein,RFP)经过一系列体外进化,其波谱范围覆盖了570～655nm,极大地丰富了细胞内或体内光学成像的荧光探针.简要阐述了来源于Discosoma sp.,Entacmaea quadricolor,Anemonia sulcata,Heteractis crispa,Actinia equina5种珊瑚的红色荧光蛋白的光学特征、结构、体外分子进化及其应用.
Red fluorescent proteins （RFPs） produced from a number ofA nthozoa species have been subjected to a series of in vitro molecular evolution, resulting in various emission spectra ranging from 570 nm to 655 nm and thus providing powerful tools for cellular imaging or even body imaging. This article briefly reviewed the optical properties, structures and mutagenesis of RFPs and their applications.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2008年第10期1112-1120,共9页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目(90606028,30700169)~~
关键词
红色荧光蛋白
分子进化
光学性质
red fluorescent protein, molecular evolution, optical property
