摘要
研究了蛇床子、苦参对茄子黄萎病菌的化感抑制作用及对茄子根际微生物数量的影响。结果表明,蛇床子、苦参的提取物抑制了茄子黄萎病菌菌丝生长,并随提取物浓度的增加抑制作用增强。在土壤中施入蛇床子、苦参提取物处理茄子苗后表现出一定的抗病效果,其中蛇床子处理的植株发病率最低。在土壤中添加蛇床子和苦参粉末,经腐解后,苦参处理的茄子植株株高、茎粗均高于对照处理,且二者处理后植株干物质含量高于对照。处理后茄子根际微生物中放线菌数量增加,真菌的数量变化差异不明显,细菌的数量相对减少,在初花期微生物的总量均高于初果期和定植期。
Eggplant verticillum wilt is one of soil-borne diseases caused by Verticillum dahliae With the development of protected and specialized vegetable production. How to control the occurrence of soil-borne plant diseases by using of the principle of allelopathy is now the focus. According to researches, plant resistances to soil-born diseases related closely to the microbial population in rhizosphere. The composition and avoiding the infection of pathogen. Fructus cnidii and Radix with their resistance to Verticillum dahliae activity. The fruit population of microorganism in rhizosphere had the effects on Sophorae Flavescentis were screened out of 54 plant extracts of Frnctus cnidii and the root of Radix Sophorae Flavescentis were used as plant materials in this research.
The results showed that extract of Fructus cnidii had inhibitory effects on mycelia growth of VerticiUum dahliae in vitro, and the higher the concentration, the greater the inhibitory effects. Treatment with concentration 20 g/L showed desired inhibitory effects and the inhibitory rate hit 79.33% after 8 days treated. Extracts of Radix Sophorae Flavescentis had positive, but less efficient effects on mycelia growth of Verticillum dahliae 8 days later the inhibitory rate with concentration 20 g/L reached 68.46%. The growth parameters of potted eggplant such as plant height, stem diameter, fresh weight above ground and fresh root weight increased significantly after treated with powder of Fructus cnidii and Radix Sophorae Flavescentis. C. monniere and S.flavescens treatments, influenced the population and composition of microorganisms in the rhizosphere. The quantity of fungi in CK was higher than that in Fructus cnidii and Radix Sophorae Flavescentis, which reached 46.52 × 10^5/g at flowering and gradually decreased at fruit stage. As for the bacterium, the highest and the lowest quantity appeared in CK and Radix Sophorae Flavescentis at flowering stage. The bacterium quantity in Fructus cnidii treatment hit 41.76 ×10^8/g at flowering stage. The quantity of actinomyces increased after treatments and reached 39.38 × 10^7/g at flowering stage. Less quantity of actinomyces was found in Cnidium monnieri than that in Radix Sophora Flavescens. It seems that allelopathic substances released by C. monnirei and S. flavescens were suppressive to Verticillium dahliae.
出处
《生态学报》
CAS
CSCD
北大核心
2008年第10期5194-5199,共6页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目(307714692)
辽宁省教育厅基金资助项目(2004D206)
沈阳农业大学校青年基金资助项目(20070213)
863 of China(No. 2004AA247010)
