摘要
目的探讨B6-Co小鼠角膜浑浊形成的细菌学因素。方法以正常B6和B6-Co小鼠为研究对象,从不同日龄组的小鼠眼部取角膜分泌物或角膜刮片,分别接种到血平板和营养肉汤上,恒温培养24h。挑取菌落或肉汤管内的悬浮液涂片,革兰氏氏染色,镜检,记录结果。将纯化培养获得的细菌进行鉴定。结果正常B6小鼠与角膜浑浊小鼠存在角膜细菌学差异。结论B6-Co小鼠角膜混浊与微生物感染有关。
Objective Keratonosus pathogen and nosogenesis of B6-Co mouse was studied to infer the cause of human keratonosus pathogeny and to control the disease. Methods Get cornea secretion or cornea scraping from the eyes of normal B6 and B6-Co mouse at different ages, and introduce them to blood plates and nutrient broth respectively, cultivate them for 24 hours at constant temperature. Pick the bacterium colonies and the suspension in the broth to prepare films, stain the films and examine them under microscope, finally identify the purified bacteria. Results There was difference in cornea bacteriology between normal B6 and B6-Co mouse. Conclusions The result showed that there was a relationship between the cloudiness of cornea and microbe infection.
出处
《实验动物与比较医学》
CAS
2008年第5期277-281,共5页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金资助项目(No:30671081)
江苏省自然科学基金资助项目(No:BK2007062)
南通大学博士科研启动基金项目(No:07B20)
