摘要
为提高拮抗菌株B36发酵生产的菌量和抗菌物质的产率,通过单因子试验和正交试验方法,对其摇床发酵培养基成分及发酵条件进行研究。结果表明,最适宜发酵条件为发酵温度28℃,转速150r·min-1,接种量20%,初始pH值9.0,在250mL三角瓶的装液量为50mL,发酵36h。最佳培养基组成为葡萄糖2.0%,酵母粉0.5%,大豆粉0.5%,NH4NO30.5%,CaCO31.0%。
The culture conditions and the compositions of cultural medium of Endophytic B6 strain isolated from Sophora with Antifungal Activity were studied by using the single factor and orthogonal design method. The optimal fermentation parameters were 28℃, 150r·min^-1＇, pH 9.0, 20% inoculation volume, and 50mL culture in 250mL flask for 36h, which had the strongest antagonistic activity. The fermentation medium was modified and the optimized medium was composed of sucrose 2.0%, yeast 0.5%, soybean flour 0.5%, NH4NO30.5% and CaCO31.0%.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2008年第5期561-564,共4页
Journal of Shenyang Agricultural University
基金
"十一五"辽宁省科技攻关项目(2006215004)
辽宁省工程技术研究中心专项(200535)
关键词
苦参
内生细菌
发酵条件
发酵培养基
正交试验
Sophoraflavescens Ait
endophytic bacteria
fermentation condition
fermentation medium
orthogonal design
