摘要
在不同低温预处理时间,不同培养基和不同激素组合下进行了苜蓿花药培养实验。结果表明,花药4℃低温预处理24 h4、8 h较72 h诱导效果好。NB培养基对愈伤组织的诱导效果比B5培养基好。最佳愈伤组织诱导培养基组合为NB+2,4-D 0.5 mg/L+NAA 0.3 mg/L+6-BA 0.5 mg/L+KT3.0 mg/L。通过不同蔗糖浓度对愈伤组织的诱导效果比较,表明低浓度的蔗糖有利于降低愈伤组织的褐化率,高浓度的蔗糖有利于单倍体愈伤组织的诱导。分化培养的最佳组合为MS+NAA 0.2 mg/L+6-BA 3.0 mg/L。诱导生根最好的培养基为1/2 MS培养基,最适合蔗糖浓度为10 g/L。
The anther of alfalfa was cultured under different conditions of medium, growth regulator formulas and temperatures. The results indicated that low temperature pretreatment for 24 to 48 hours was better than that of treatment for 72 hours for inducing callus. NB medium supplemented with 2,4-D(0.5 mg/L),NAA (0.2 mg/L),6-BA (0.5 mg/L) and KT (3 mg/L) was the best for induction of callus. MS + NAA (0.2 mg/L)+ 6-BA (3.0 mg/L) was the best for regeneration culture. The higher level of sucrose was suitable for the induction of haploid callus,while the lower level could reduce the browning frequency.
出处
《草原与草坪》
CAS
2008年第5期1-5,共5页
Grassland and Turf
基金
草业生态系统教育部省部共建重点实验室基金项目
甘肃农业大学科技创新项目
甘肃省教育厅科学技术研究项目"豆科模式植物蒺藜苜蓿作图群体的构建"
国家自然科学基金(30671486)资助
关键词
苜蓿
花药培养
倍性鉴定
Alfalfa
anther cult ure
chromosome counting identification