摘要
以新西伯利亚银白杨叶片为外植体,建立了再生体系。经正交试验筛选出此组织培养的最适外植体产生不定芽诱导培养基为MS+6-BA0.1 mg.L-1+GA0.1 mg.L-1+NAA0.05 mg.L-1,最佳不定芽生根培养基为1/2MS+NAA0.1 mg.L-1+IBA0.02 mg.L-1。
l.eaves of Populus hybride were used as explants to set up regeneration system. The optimal medium formula （ MS ＋ 6-BA 0.1 mg ·L^-1 ＋ GA 0. 1 mg ·L^-1 ＋ NAA 0.05 mg·L^-1） which is suitable to adventitious bud induction for explant was screened out by orthogonal test. The optimal medium of root regeneration from adventitious bud is 1/2 MS ＋ NAA 0.1 mg·L^-1 ＋ IBA 0.02 mg ·L^-1.
出处
《防护林科技》
2008年第6期25-26,共2页
Protection Forest Science and Technology
基金
黑龙江省科技计划项目(GB06B303-7J)
