摘要
目的:建立复方扶芳藤胶囊(红参,黄芪、扶芳藤等)中卫矛醇和黄芪甲苷的含量测定方法。方法:采用正相HPLC法,色谱柱:Lichrospher5-NH2柱(250mm×4.6mm,5μm);流动相:乙腈-水(91∶9);流速1.0mL/min;柱温为28℃;检测器:PL-ELS2100型蒸发光散射检测器(漂移管温度:65℃,雾化室温度:50℃,气体流量:0.9L/min)。结果:卫矛醇进样量在2.91～29.1μg范围内,黄芪甲苷在1.03～10.3μg范围内,其峰面积的自然对数(Y)与进样量的自然对数(X)呈良好的线性关系(r>0.9990),卫矛醇和黄芪甲苷的平均加样回收率分别是99.24%和103.17%,RSD分别为2.6%和1.8%(n=6)。结论:该方法稳定、重复性好,结果准确,可作为复方扶芳藤胶囊中卫矛醇和黄芪甲苷的含量测定方法。
AIM： To establish an HPLC-ELSD method for determing dulcitol and astragaloside in Compound Fufangteng Capsules （Radix et Rhizoma Ginseng rubra, Radix Astragali, Herba Euonymi, etc）. METHOD： Chromatographic conditions included Lichrospher 5-NH2 column （250 mm×4.6 mm, 5μm） and the mobile phase consisting of a mixture of acetonitrile-water （91：9）. The flow rate of mobile phase was 1.0 mL/min. The column tempe-rature was at 28℃. Detector： PL-ELS2100 ELSD （ Eva ： 65 ℃, Ned ： 50 %, gas flow ： 0.9 L/min）. RESULTS： The linear ranges of dulcitol and astragaloside were 2.91 -29.1 μg （r = 0. 999 8 ） and 1.03 - 10.3μg （ r = 0. 999 1 ） , respectively. The average recoveries of dulcitol and astragaloside were 99.24% and 103.17% with corresponding RSD of 2.6 % and 1.8% （ n = 6） , respectively. CONCLUSION ： The method is steady and with good repeatability, and can be used to determine the content of dulcitol and astragaloside in Compound Fufangteng Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2008年第10期1468-1471,共4页
Chinese Traditional Patent Medicine
基金
广西自然科学基金资助项目(桂科自0447076)
