摘要
目的测定冠心丹参滴丸中丹酚酸B的含量。方法HPLC法，ODS2-C18色谱柱（250mm×4．6mm，5μm），流动相：（甲醇-乙腈）：（甲酸-水）=37：63，其中甲醇：乙腈=3：1，甲酸：水=1：29；流速1．0ml·min^-1；检测波长为286nm。结果丹酚酸B浓度在0．1—0．9μg范围内线性关系良好（r=0．9995，n=5）。加样回收率为101．56％，RSD=2．86％（n=6）。结论该实验提取丹酚酸B处理方法简单，测定结果稳定可靠，可作为冠心丹参滴丸中丹酚酸B的定量分析方法.
Objective To quantitatively determine salvianolic acid B in Guanxindanshen Drop pills. Methods The HPLC procedure was used in the detetvaination, the chromatographic column was ODS2 - C18 （250 mm ×4.6 mm, 5μm）, the mobile phase was （methanol- acetonitrile）： （formic acid-water） =37： 63, and methanol：acetonitrile =3： 1, formic acid： water = 1： 29. The flow velocity was 1.0 ml · min^- 1 , and the detection wavelength was 286 nm. Results It showed that salvianolic acid B had a good linear relationship when its concentration was in the range of 0.1 - 0.9gg（ r =0. 999 5, n = 5 ） ; and the sample recovery of the procedure was 101.56% （RSD = 2.86%, n = 6）. Conclusion The method is simple and accurate. It can be used for extraction and determination of salvianolic B in Guanxindanshen Drop pills.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第10期2439-2440,共2页
Lishizhen Medicine and Materia Medica Research
