摘要
目的探讨研究弓形虫淋巴结炎的病理诊断。方法应用免疫组化、原位杂交及间接原位PCR技术，检测71例（均来自可疑弓形虫感染者）经病理诊断为慢性非特异性淋巴结炎（淋巴结反应性增生）病人组织切片中的弓形虫。结果发现在71例中弓形虫阳性病例分别为17例（23．94％）、35例（49．30％）、41例（57．75％），免疫组化与原位杂交、间接原位PCR结果差异有统计学意义（P〈0．005）。结论在病理诊断为慢性非特异性淋巴结炎（淋巴结反应性增生）的病人中，部分为漏诊的弓形虫淋巴结炎病例。免疫组化、原位杂交及间接原位PCR均能显示淋巴结组织切片中的弓形虫，但弓形虫检测结果阳性率从高到低依次为间接原位PCR、原位杂交及免疫组化。
Objective Research the pathologic approaches to diagnose toxoplasmic lymphnoditis. Methods Appling immunohistochemistry, in-suit hybridization and indirect in-suit PCR technology to detect 71 cases of Toxoplasma gondii, these cases of tissue slices are pathologicaly diagnosed as chronic nonspecific lymphadenitis. Results 17 cases is positive among 71 cases with immunohistochemistry, while with in-suit hybridization ,35 cases show positive ,the techonology of indirect in-suit PCR has the most positive cases, that is 41 cases. It has statistic significance among the three methods （P〈0.005）. Conclusions In the cases that are pathologicaly diagnosed as chronic nonspecific lymphadenitis, some of them are failed to be detected as toxoplasmic lymphadenitis. It has different sensitivity among these diagnose approaches, indirect in-suit PCR is the most sensitive, in-suit hybridization is lower, while immunohistochemistry is the least one.
出处
《世界感染杂志》
2008年第5期375-378,共4页
World Journal of Infection
关键词
弓形虫
淋巴结
免疫组化
原位杂交
间接原位PCR
Toxoplasma gondii
Lymphadenode
Immunohistochemistry
In suit hybridization
Indirect in-suit PCR
