摘要
对姜花属的SRAP-PCR反应体系进行了Mg离子、dNTPs、Taq酶、引物以及模板DNA浓度等方面的优化,除Mg离子浓度外,优化后的体系在其它各成分上,与原始体系相比,均显著降低了用量,节约了成本。并用优化后的体系对姜科其它14个属进行了扩增,均可得到良好的扩增效果,而且揭示的多态性很高,说明SRAP标记可以用来进行姜科各属内及属间分类和亲缘关系分析。
The system of SRAP in Hedychium was optimized including the concentration of Mg^2＋ , dNTPs, TaqE, primers and DNA template etc. Compared with original system,this system reduced notably the consumption of all components except Mg^2＋. Amplified reactions of other fourteen genera in Zingiberaceae with this system appeared clear bands and high polymorphism. The result showed that SRAP maker can be used in taxonomy and genetic connection analysis of varieties, species or genera in Zingiberaceae.
出处
《广西植物》
CAS
CSCD
北大核心
2008年第5期604-607,共4页
Guihaia
基金
广东省科技攻关项目(2006B20201044)~~
关键词
姜花属
SRAP
优化
分类
亲缘关系分析
Hedychium
SRAP
optimize
taxonomy
genetic connection analysis
