摘要
目的：建立HPLC法测定尿素咪康唑乳膏中硝酸咪康唑的含量。方法：采用Diamonsil C18柱，流动相为0．1％乙酸铵溶液：甲醇（10：90），流速1．0mL／min，检测波长240nm，以苯甲酸苄酯为内标。结果：硝酸咪康唑在300-500μg／mL范围内线性关系良好（r=0．9999），回收率为99．62％～101．6％，日内、日间RSD均〈1．5％。结论：此法快速、准确、灵敏、简便，能消除杂质干扰，适合于尿素咪康唑乳膏中硝酸咪康唑的含量测定。
Objective：To establish a HPLC method for determination of content of miconazole nitrate in urea and miconazole cream. Methods：A Diamonsil C18 column was used with the mobile phase of 0.1% ammonium acetate solution-methanol （10 ： 90） at the detection wavelength of 240 nm. The flow rate was 1.0 mL/min. Benzyl benzoate was used as internal standard. Results：The calibration curve of miconazole nitrate was linear in the range of 300.0-500.0μg/mL（r=0. 999 9）. The reco- very was 99.62 %-101.6 %. The intra and inter-day RSD was less than 1.5 %. Conclusion： This method is rapid, accurate, sensitive, simple and can avoid the interference. It can be employed for the determination of the concentration of mieonazole nitrate in urea and mieonazole cream.
出处
《药学服务与研究》
CAS
CSCD
2008年第5期367-369,共3页
Pharmaceutical Care and Research
关键词
尿素咪康唑乳膏
硝酸咪康唑
色谱法
高压液相
苯甲酸苄酯
urea and miconazole cream
miconazole nitrate
chromatography, high pressure liquid
benzyl benzoate
