摘要
背景与目的:目前,对于肿瘤坏死因子样凋亡微弱诱导剂(TNF-like weak inducer of apoptosis,TWEAK)在乳腺癌中的表达存在争议,本研究探讨TWEAK在乳腺癌组织及不同侵袭能力乳腺癌细胞系中表达情况及其与微血管密度(microvessel density,MVD)的关系。方法:应用免疫组化SP法检测TWEAK在70例乳腺癌组织和30例癌旁非癌乳腺组织中的表达。应用Western blot法检测TWEAK在乳腺癌细胞系MCF-7(低转移)和MDA-MB-231(高转移)中的表达。应用ELISA方法检测TWEAK在乳腺癌细胞系MCF-7和MDA-MB-231中的分泌情况。结果:TWEAK在乳腺癌组织中表达(60%)明显高于癌旁非癌组织(6.67%),且在浸润性导管癌组织(76.67%)中表达明显高于导管内癌组织(42.85%,P=0.003)。浸润性导管癌组织中MVD高于导管内癌(P<0.05)。浸润性导管癌组织中TWEAK表达与MVD明显相关(r=0.611),导管内癌中不显著(r=0.015)。MDA-MB-231细胞系表达TWEAK明显高于MCF-7细胞系(t=4.259,P=0.007)。MDA-MB-231细胞系分泌可溶性TWEAK高于MCF-7细胞系(t=3.6504,P=0.006)。结论:TWEAK的表达与乳腺癌侵袭能力相关。
BACKGROUND ＆ OBJECTIVE： The expression of TNF-like weak inducer of apoptosis （TWEAK） in breast cancer remains disputable. This study was to investigate the expression of TWEAK in breast cancer tissues and breast cancer cell lines with different invasive abilities, and the relationship of TWEAK with microvessel density （MVD）. METHODS： Immunohistochemical S-P method was adopted to detect the expression of TWEAK in 70 specimens of breast cancer and 30 specimens of adjacent normal breast tissues. The protein expression of TWEAK was determined by Western blot in a poorly invasive breast cancer cell line MCF-7 and a highly invasive breast cell line MDA-MB-231. Secretion of TWEAK was measured by ELISA assay in MCF-7 and MDA-MB-231 cells. RESULTS： The expression of TWEAK was higher in breast cancer （60%） than in adjacent normal breast tissues（6.67%） （P〈0.05）, and is higher in infiltrating ductal carcinoma of the breast （76.67 %） than in breast ductal carcinoma in situ （42.85%） （P= 0.003）. MVD was higher in infiltrating ductal carcinoma of the breast than in breast ductal carcinoma in situ （P〈0.05）. The expression of TWEAK was significantly correlated with MVD in infiltrating ductal carcinoma of the breast （r=0.611）, but not with breast ductal carcinoma in situ （r=0.015）. The expression of TWEAK and secretion of soluble TWEAK were higher in MDA- MB-231 cells than in MCF-7 cells （t=4.259, P=0.007; t=3.6504, P=0.006 ）. CONCLUSION： TWEAK expression is related to the metastatic ability of breast cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第11期1177-1181,共5页
Chinese Journal of Cancer
关键词
TWEAK
乳腺肿瘤
微血管密度
TNF-like weak inducer of apoptosis （TWEAK）, breast neoplasm,microvessel density （MVD）
