摘要
采用聚合酶链式反应/变性梯度凝胶电泳技术(PCR-DGGE),研究了盆栽条件下,接种多粘类芽孢杆菌(Paenibacillus polymyxa)和枯草芽孢杆菌(Bacillus subtilis)对不同时期黄瓜和番茄根际土壤微生物群落结构的影响。通过DGGE图谱的主成分分析表明,这2株生防菌对根际微生物的影响因作物的种类不同而不同:对黄瓜根际细菌和真菌群落结构均无显著影响,真菌群落随着取样时期而发生明显变化;对番茄根际细菌群落结构无明显影响,但不同采样时期细菌群落结构发生明显变化,而2株生防菌对其真菌群落结构的变化有显著影响。蔬菜种类是决定根际微生物群落结构的主要因素,接种生防细菌对根际细菌群落结构影响不显著,而对根际真菌群落结构的影响因蔬菜种类的不同而有差异。
In a pot experiment, the effects of bio-control bacterial strains Paenibacillus polymyxa and Bacillus subtilis on the microbial community structure in the rhizosphere soils of cucumber and tomato were examined by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. Principal component analysis （PCA） of DGGE banding patterns indicated that the effects of the two bacillus strains varied with cropped plants. In the rhizosphere soil of cucumber, no significant effects were observed on the structures of bacterial and fungal communities, but that of fungal community changed obviously with sampling time ; while in the rhizosphere soil of tomato, the structure of bacterial community was less affected but changed obviously with sam- piing time, and the structure of fungal community was significantly affected. It was suggested that vegetable species was the major factor determining the microbial community structure in rhizosphere soil. Inoculation with test two bio-control bacterial strains had little effects on the structure of bacterial community, but its effects on fungal community structure differed with vegetable species.
出处
《生态学杂志》
CAS
CSCD
北大核心
2008年第11期1895-1900,共6页
Chinese Journal of Ecology
基金
哈尔滨市科技攻关资助项目(2004AA3CN154)
