摘要
【目的】研究AM菌剂接种对酿酒葡萄赤霞珠(Cabernet sauvignon)生长和耐旱性的影响,为AM菌剂在生产实践中的推广应用提供理论依据。【方法】在盆栽试验条件下,用摩西球囊霉(Glomus mosseae,简称Gm)、幼套球囊霉(Glomus etunicatum,简称Ge)和Gm+Ge混合菌剂接种处理赤霞珠葡萄扦插苗,以接种高温灭菌的等量菌剂为对照,于接种处理100 d后,测定菌剂接种处理对赤霞珠植株的侵染率和营养生长的影响。随后进行了30 d的干旱胁迫,对不同菌剂处理后葡萄扦插苗的抗旱性进行了比较。【结果】接种AM菌剂能显著提高赤霞珠葡萄的生长量,并且菌根依赖性与赤霞珠根系菌根侵染率呈显著正相关;在干旱胁迫下,赤霞珠叶片中的SOD、POD和CAT 3种保护酶活性显著升高,MDA含量和相对电导率降低,赤霞珠的耐旱性显著提高。【结论】3种AM菌剂处理的效应由小到大依次为Gm<Ge<Gm+Ge,AM混合菌剂接种是值得研究与推广应用的一种菌根真菌利用方式。
[Objective] The growth and the impact of drought tolerance of Cabernet Sauvignon after inoculating AM （Arbuscular mycorrhizal） fungi was studied in this experiment in order to provide theoretical basis for promoting the ues of AM fungi in production. [Method] Cutting seedlings were inoculated by Glomus mosseae （Gm） ,Glomus etunicatum （Ge） and the mixture of Ge and Gm in the pot and the control were treated by the same AM inoculants after disinfecting through high-temperature. The mycorrhizal colonization rate and the grape＇s growth were determinated after 100 d,then drought resistance of different cutting seedlings were compared with each other after drought stress for 30 d. [Result] AM fungi inoculation significantly enhanced the growth of Cabernet sauvignon,and Mycorrhizal dependency was correlated with the mycorrhizal colonization significantly. The activity of three protective enzymes,SOD,CAT and POD of Cabernet Sauvignon leaves, increased significantly, MDA content and relative conductivity decreased, and the drought tolerance of Cabernet Sauvignon was remarkably improved during drought stress. [Conclusion] The effect order of three AM fungi inoculants was Gm〈Ge〈Gm＋Ge. The mixture of AM fungi inoculants was an effective quomodo for the ectomycorrhizal fungi utility.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2008年第11期191-196,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省科技攻关项目(2004K02-G9)
关键词
AM菌剂
赤霞珠
扦插苗
生长量
抗旱性
AM fungi
Cabernet sauvignon
cutting seedling
growth amount
drought resistance
